内蒙古呼伦贝尔地区的可培养黏细菌及其抗菌活性

doi:10.13560/j.cnki.biotech.bull.1985.2019-0210

摘要/Abstract

摘要： 从内蒙古呼伦贝尔地区采集土壤样品,测定其环境参数,采用大肠杆菌划线、兔粪诱导及滤纸诱导3种方法对20份土壤样品进行黏细菌的分离纯化及鉴定,再通过平板对峙法确定其抗菌活性,最后用统计学方法进行分析。结果表明,呼伦贝尔地区土样整体呈酸性,含水量较低,且土壤养分含量不均衡。共得到了41株黏细菌,分属于4个属,包括Myxococcus、Corallococcus、Cystobacter和Archangium,优势菌属为Myxococcus。在土壤类型为黑土以及利用方式为草地的样品中黏细菌表现出较丰富的多样性。黏细菌分布与环境因子之间未表现出显著的相关性。所有黏细菌均抑制马铃薯晚疫病菌生长且溶解大肠杆菌,对枯草芽孢杆菌、酿酒酵母菌、核盘菌、立枯丝菌和尖孢镰刀菌均无抑制作用,有3株抑制金黄色葡萄球菌的生长。这些研究结果说明内蒙古呼伦贝尔地区的可培养黏细菌资源比较丰富且具有显著的抗马铃薯晚疫病菌活性,为该地区黏细菌资源的深入研究及开发提供了基础数据。

关键词: 呼伦贝尔, 黏细菌, 环境参数, 分离鉴定, 抗菌活性, 相关性分析

Abstract: Total 20 soil samples were collected from Hulun Buir,Inner Mongolia and their environmental parameters were measured. Myxobacteria were isolated from 20 soil samples and then purified and identified by Escherichia coli-inducing method,the rabbit dung pellets-inducing method and filter paper-inducting method. Then the antibiotic activities of the myxobacterial strains were determined by the plate confrontation method,and the results were analyzed by statistical methods. The results showed that the soil nutrient content was unbalanced in Hulun Buir,the soil samples were generally acidic,and the soil water content was low. A total of 41 strains of myxobacteria were obtained,belonging to Myxococcus,Corallococcus,Cystobacter and Archangium;and the dominant one was Myxococcus. The strains showed rich diversity in black soil and grassland. There was no significant correlation between the strains and environmental factors. All the strains presented activity against E. coli and Phytophthora infestans,3 strains inhibited Staphylococcus aureus,but they show no inhibitory effect on Bacillus subtilis,Saccharomyces cerevisiae,Sclerotinia sclerotiorum,Rhizoctonia solanikuhn,and Fusarium oxysporum. In conclusion,the cultivable myxobacteria are abundant in Hulun Buir,and all of them demonstrated obvious activity against P. infestans,providing basic data for the research and development of myxobacteria resources in Hulun Buir,Inner Mongolia.

Key words: Hulun Buir, myxobacteria, environmental parameters, isolation and identification, antibiotic activity, correlation analysis

王雪寒, 马强, 田媛, 胡靖, 刘惠荣. 内蒙古呼伦贝尔地区的可培养黏细菌及其抗菌活性[J]. 生物技术通报, 2019, 35(9): 224-233.

WANG Xue-han, MA Qiang, TIAN Yuan, HU Jing, LIU Hui-rong. Cultivable Myxobacteria and Their Antibiotic Activities in the Hulun Buir Area of Inner Mongolia[J]. Biotechnology Bulletin, 2019, 35(9): 224-233.

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