稻瘟病菌精氨酸甲基转移酶基因MoHMT1的功能分析

doi:10.13560/j.cnki.biotech.bull.1985.2018-0313

摘要/Abstract

摘要： 由蛋白质精氨酸甲基转移酶（Protein arginine methyltransferases,PRMTs）调控的精氨酸甲基化是真核生物中普遍存在的翻译后修饰。为研究稻瘟病菌MoHMT1（hnRNP arginine N-methyltransferase,PRMT1的同源蛋白）基因的功能,本研究根据同源重组的原理获得Mohmt1的敲除突变体,并对突变体进行了初步的表型分析。结果表明,与野生型相比,MoHMT1基因的缺失导致稻瘟病菌的菌落变小,表面气生菌丝变薄,生长速率明显减慢。虽然MoHmt1突变体的产孢量明显下降,仅为野生型的20%左右,但是产生的分生孢子能正常萌发产生附着胞,并成功侵入洋葱表皮细胞。进一步利用孢子悬浮液接种水稻叶片,发现Mohmt1缺失突变体病斑数显著减少,致病能力减弱。以上实验结果表明MoHMT1蛋白可能在稻瘟病菌菌丝生长发育和侵染致病过程中发挥了重要的调控作用。

关键词: 稻瘟病菌, MoHMT1, 基因敲除, 生长发育, 致病性

Abstract: Arginine methylation regulated by protein arginine methyltransferases（PRMTs）is a common post-translational modification in eukaryotic organisms. In order to analyze the functions of gene MoHMT1（hnRNP arginine N-methyltransferase,PRMT1 homologous）,Mohmt1-knockout mutants were obtained based on the principle of homologous recombination,and preliminarily phenotypic characterizations were carried out. The results showed that the deletion of Mohmt1 resulted in the smaller colony,thinner aerial hyphae,and significantly slower growth of Magnaporthe oryzae,compared to the wild type. The conidia amount produced by Mohmt1-deleted mutants demonstrated obviously reduction with only 20% of the wild type;however,the conidia produced by the mutants normally germinated to form appressorium and successfully penetrated the onion epidermal cells. Furthermore,using the conidia suspension to inoculate the tested rice Leaves,it was found that the number of Lesions produced by the MoHmt1-deleted mutants significantly decreased related to the wild type and deletion of MoHmt1 attenuated pathogenicity on susceptible rice. These results indicate that MoHMT1 protein may play important regulation role in the hyphal development and pathogenic process of M. oryzae.

Key words: Magnaporthe oryzae, MoHMT1, gene knockout, growth and development, pathogenicity

张文泽, 张艳丽, 门彦明, 张玉娇, 孙志昕, 李文慧, 鲁国东, 齐尧尧. 稻瘟病菌精氨酸甲基转移酶基因MoHMT1的功能分析[J]. 生物技术通报, 2019, 35(12): 38-44.

ZHANG Wen-ze, ZHANG Yan-Li, MEN Yan-ming, ZHANG Yu-jiao, SUN Zhi-xin, LI Wen-hui, LU Guo-dong, QI Yao-yao. Functional Analysis of Arginine N-methyltransferase Gene MoHMT1 in Magnaporthe oryzae[J]. Biotechnology Bulletin, 2019, 35(12): 38-44.

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