生物技术通报 ›› 2019, Vol. 35 ›› Issue (12): 45-49.doi: 10.13560/j.cnki.biotech.bull.1985.2019-0263

• 研究报告 • 上一篇    下一篇

Bacillus amyloliquefaciens YM6对盐胁迫条件下玉米促生长作用研究

王华笑1, 刘环1, 杨国平1, 2, 3, 张琇1, 2, 3, 李壮1, 张炎4   

  1. 1. 北方民族大学生物科学与工程学院,银川 750021;
    2. 宁夏特殊生境微生物资源开发与利用重点实验室,银川 750021;
    3. 国家民委发酵酿造工程生物技术重点实验室,银川 750021;
    4. 新疆农业科学院土壤肥料与农业节水研究所,乌鲁木齐 830091
  • 收稿日期:2019-04-01 出版日期:2019-12-26 发布日期:2019-12-03
  • 作者简介:王华笑,男,研究方向:微生物生态学;E-mail:1147289657@qq.com
  • 基金资助:
    宁夏科技支撑计划项目重大专项(宁科技字(2015)26号),北方民族大学创新项目(YCX19104)

Effect of Bacillus amyloliquefaciensYM6 on Growth Promotion of Maize Under Salt Stress

WANG Hua-xiao1, LIU Huan1, YANG Guo-ping1, 2, 3, ZHANG Xiu1, 2, 3, LI Zhuang1, ZHANG Yan4   

  1. 1. College of Biological Science & Engineering,North Minzu University,Yinchuan 750021;
    2. Ningxia Key Laboratory for Development and Application of Microbial Resources in Extreme Environment,Yinchuan 750021;
    3. Key Laboratory of Fermentation,Brewing Engineering and Biotechnology,State Nationalities Affairs Commission,Yinchuan 750021;
    4. Research Institute of Soil,Fertilizer and Agricultural Water Conservation,Xinjiang Academy of Agricultural Sciences,Urumqi 830091
  • Received:2019-04-01 Published:2019-12-26 Online:2019-12-03

摘要: 以解淀粉芽孢杆菌YM6(Bacillus amyloliquefaciensYM6)为对象,研究其在盐胁迫条件下对玉米的促生效果,为提高盐碱地作物产量开发新型菌源。利用水培试验,设置0、25、50、75和100 mmol/L NaCl的植物营养液,培养玉米植株,同时接种YM6菌株,观察其对盐胁迫下玉米生长的影响。结果表明,在75 mmol/L的盐胁迫条件下,与盐胁迫对照组相比接种YM6菌株组的玉米幼苗株高增加15.18%、根长增加28.80%,植株干重增加27.01%。在盐碱土盆栽试验中,接种YM6菌株组的玉米株高比未接种YM6组增加37.8%,植株干重增加43.9%。此外,YM6菌株能够在玉米根系定殖。初步研究表明YM6菌株可有效缓解盐胁迫对玉米生长的不利影响,促进玉米生长。

关键词: 解淀粉芽孢杆菌, 玉米, 盐胁迫, 促生作用

Abstract: Bacillus amyloliquefaciens strain YM6 isolated from soil is able to mitigate the salt stress on maize. More detailed effects of stress relieving by this strain need to be elucidated. Provide new bacterials for the production of non-halophyte such as Maize and the improvement of saline soils in related areas. Maize seedlings were growing in hydroponic jars containing plant nutrients solution amended different concentrations of NaCl. YM6 was inoculated to these salt stressed seedlings,and plant growth was measured and analyzed. Results showed under 75 mmol/L NaCl stress,compared to the un-inoculated control,plant height increased by 15.2% when YM6 was inoculated to roots of maize seedlings. Root length and plant dry weight of inoculated maize increased by 28.8% and 27.0% respectively as compared to un-inoculated control. In the bonsai experiments with saline-alkali soil,plant height and dry weight of maize treated with YM6 increased by 37.8% and 43.9% respectively as compared to the un-inoculated control. Strain YM6 was also found to colonize maize root.Our preliminary results indicated that YM6 is able to alleviate the adverse effects of salt stress on maize.

Key words: Bacillus amyloliquefaciens, maize, salt stress, growth promotion