RNA干扰技术在水产动物抗病毒和抗寄生虫研究中的应用研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2019-0908

摘要/Abstract

摘要： RNA干扰（RNA interference,RNAi）是由双链RNA介导的,抑制目标基因的表达,沉默靶基因的一种转录后基因沉默机制,并且在真核生物中广泛存在。近年来随着水产养殖业的发展壮大,水产动物疾病频繁爆发,给养殖户带来巨大的经济损失。目前,病毒、寄生虫等病原引起的水产动物疾病的致病机制还有待深入研究。RNA干扰技术的出现为水产动物疾病致病机制的研究提供了强有力的工具。主要对 RNA 干扰的发现、作用机制以及在水产动物抗病毒和抗寄生虫研究中的应用作以综述,并对未来RNAi技术在水产动物疾病防治中的研究和应用进行了展望,旨为水产动物疾病控制提供参考。

关键词: RNA干扰, 水产动物, 抗病毒, 抗寄生虫, 应用

Abstract: RNA interference（RNAi）is a post-transcriptional gene silencing mechanism mediated by double-stranded RNA which inhibits the expression of target genes,and is widely found in eukaryotes. With the development of aquaculture in recent years,diseases of aquatic animals often break out,bringing huge economic losses to farmers. Currently,the mechanism of aquatic animal diseases caused by viruses and parasites still needs to be further studied. The emergence of RNA interference technology provides a powerful tool for studying the pathogenic mechanism of aquatic animal diseases. In this paper,the discovery,mechanism and application of RNA interference in the antiviral and antiparasitic research of aquatic animals are reviewed,and the future research and application of RNAi technology in the prevention and treatment of aquatic animal disease are prospected,providing reference for the control of diseases in aquatic animals.

Key words: RNA interference, aquatic animals, antiviral, antiparasitic, application

宋华丽, 孙效迎, 孔祥会, 李莉, 裴超. RNA干扰技术在水产动物抗病毒和抗寄生虫研究中的应用研究进展[J]. 生物技术通报, 2020, 36(2): 193-205.

SONG Hua-li, SUN Xiao-ying, KONG Xiang-hui, LI Li, PEI Chao. Application of RNA Interference Technology in Antiviral and Antiparasitic Research of Aquatic Animals[J]. Biotechnology Bulletin, 2020, 36(2): 193-205.

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