生物技术通报 ›› 2020, Vol. 36 ›› Issue (3): 54-61.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0008
• 基因编辑专题(专题主编 谷峰 研究员) • 上一篇 下一篇
韩梅, 袁超, 郭婷婷, 吴怡, 岳耀敬, 杨博辉
收稿日期:
2020-01-03
出版日期:
2020-03-26
发布日期:
2020-03-17
作者简介:
韩梅,女,硕士研究生,研究方向:动物遗传育种与繁殖;E-mail:82101186189@caas.cn
基金资助:
HAN Mei, YUAN Chao, GUO Ting-ting, WU Yi, YUE Yao-jing, YANG Bo-hui
Received:
2020-01-03
Published:
2020-03-26
Online:
2020-03-17
摘要: 体细胞核移植技术(Somatic cell nuclear transfer,SCNT)基本上分为传统克隆(Traditional cloning,TC)和手工克隆(Handmade cloning,HMC)。虽然随着SCNT的推进,科研人员已经利用TC成功克隆出多种动物,但这种方法需要使用昂贵的显微操作仪,并且克隆效率较低。因此,TC逐渐被新方法HMC替代,HMC是一种通过手工操作将体细胞与两个去核的半卵母细胞融合、激活,并在培养系统中培养后产生胚胎的方法。HMC不仅操作程序简单、不需要显微操作仪、成本较低,还可以提高囊胚率,使克隆技术向生产应用又迈进了一大步。主要对HMC的操作程序、表观遗传重编程异常和HMC重编程异常修复进行了综述,并对HMC进行了展望,以期能够更好地将其应用到生产中去。
韩梅, 袁超, 郭婷婷, 吴怡, 岳耀敬, 杨博辉. 哺乳动物手工克隆的研究进展[J]. 生物技术通报, 2020, 36(3): 54-61.
HAN Mei, YUAN Chao, GUO Ting-ting, WU Yi, YUE Yao-jing, YANG Bo-hui. Research Progress on Handmade Cloning in Mammalian[J]. Biotechnology Bulletin, 2020, 36(3): 54-61.
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