CRISPR /Cas9基因编辑技术在山羊和绵羊中的应用研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2020-0068

生物技术通报 ›› 2020, Vol. 36 ›› Issue (3): 62-68.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0068

• 基因编辑专题（专题主编谷峰研究员） • 上一篇下一篇

CRISPR /Cas9基因编辑技术在山羊和绵羊中的应用研究进展

宋绍征¹, 陆睿², 张婷³, 何正义³, 吴赵曼秋¹, 成勇³, 周鸣鸣¹

1. 无锡太湖学院护理学院,无锡 214000;
2. 江苏食品药品职业技术学院,制药工程学院,淮安 223003;
3. 扬州大学兽医学院江苏省转基因动物制药工程研究中心,扬州 225009

收稿日期:2020-01-17 出版日期:2020-03-26 发布日期:2020-03-17
作者简介:宋绍征,男,博士,讲师,研究方向：转基因与胚胎工程;E-mail：ssz0610@163.com;陆睿同为本文第一作者
基金资助:
江苏省高校自然科学基金面上项目（19KJB180030）,江苏高校哲学社会科学研究一般项目（2019SJA0808）,无锡市科协软科学研究重点课题（KX-19-B28）,无锡太湖学院横向课题（17WURD009）

Research Progress of CRISPR /Cas9 Gene Editing Technology in Goat and Sheep

SONG Shao-zheng¹, LU Rui², ZHANG Ting³, HE Zheng-yi³, WU Zhao-manqiu¹, CHENG Yong³, ZHOU Ming-ming¹

1. School of Nursing,Wuxi Taihu University,Wuxi 214000;
2. School of Pharmaceutical Engineering,Jiangsu Food and Pharmaceutical Science College,Huai’an 223003;;
3. College of Veterinary Medicine/Jiangsu Provincial Research Center for Animal Transgenesis and Biopharming,Yangzhou University,Yangzhou 225009

Received:2020-01-17 Published:2020-03-26 Online:2020-03-17

摘要/Abstract

摘要： CRISPR /Cas9系统是近年来新兴的一种基因编辑技术,可将特定DNA基因序列敲除、插入或定点突变,具有快捷、高效、精准、特异性高等特点,广泛地应用于遗传育种、生物医药和基因工程等研究领域。山羊和绵羊是重要的经济家畜动物和实验动物,利用CRISPR/Cas9基因编辑系统对羊进行遗传修饰,将加速品种改良,提高动物生产性能,获得更加优质的农副产品。主要对CRISPR /Cas9基因编辑技术的概述、作用机理及在羊乳“人源化”改造、提高肉品质、改善毛纤维质量等方面的应用研究进展及发展前景作简要阐述,以期为相关科研人员提供参考。

关键词: CRISPR /Cas9, 基因编辑, 基因组, 遗传, 山羊, 绵羊

Abstract: CRISPR/cas9 system is a new gene editing technology in recent years,and it can precisely edit and modify the specific DNA sequence of organism,including knockout,insertion or site-directed mutation. It is simple,efficient,precise and specific. Therefore,it has been widely used in biomedicine,gene engineering and genetic breeding research. The goat and sheep are important economic domestic animal and experimental animal. Genetic modification of sheep and goat by CRISPR/cas9 editing system can accelerate breed improvement,increase livestock production and obtain high quality agricultural and sideline products. Here we mainly summarize the overview,mechanism and the research progress of CRISPR/cas9 gene editing technology and its application in “humanized” modification of goat milk,raising meat quality,improving wool fiber quality,and the prospect,aiming at providing reference for relevant researchers.

Key words: CRISPR/cas9, gene editing, genome, genetics, goat, sheep

宋绍征, 陆睿, 张婷, 何正义, 吴赵曼秋, 成勇, 周鸣鸣. CRISPR /Cas9基因编辑技术在山羊和绵羊中的应用研究进展[J]. 生物技术通报, 2020, 36(3): 62-68.

SONG Shao-zheng, LU Rui, ZHANG Ting, HE Zheng-yi, WU Zhao-manqiu, CHENG Yong, ZHOU Ming-ming. Research Progress of CRISPR /Cas9 Gene Editing Technology in Goat and Sheep[J]. Biotechnology Bulletin, 2020, 36(3): 62-68.

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CRISPR /Cas9基因编辑技术在山羊和绵羊中的应用研究进展

Research Progress of CRISPR /Cas9 Gene Editing Technology in Goat and Sheep

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