生物技术通报 ›› 2021, Vol. 37 ›› Issue (6): 66-72.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0473

• 研究报告 • 上一篇    下一篇

猕猴桃溃疡病菌拮抗菌的筛选、鉴定及其对植物病原真菌的抗性

朱海云(), 马瑜(), 柯杨, 李勃   

  1. 陕西省微生物研究所,西安 710043
  • 收稿日期:2020-04-22 出版日期:2021-06-26 发布日期:2021-07-08
  • 作者简介:朱海云,女,硕士,研究方向:应用生物技术;E-mail: zhuhaiyun@ms.xab.ac.cn
  • 基金资助:
    西安市科技计划项目(20193066YF054NS054);陕西省科学院科技计划项目(2018nk-03)

Screening and Identification of an Antagonist Against the Pathogen of Kiwifruit Canker and Its Antifungal Activity to the Phytopathogenic Fungus

ZHU Hai-yun(), MA Yu(), KE Yang, LI Bo   

  1. Shaanxi Microbiology Institute,Xi'an 710043
  • Received:2020-04-22 Published:2021-06-26 Online:2021-07-08

摘要:

从野生银杏中分离、筛选抗猕猴桃溃疡病内生细菌,并明确其对常见植物真菌病害的抑菌谱。采用“三步法”分离银杏内生细菌,并以猕猴桃溃疡病病原菌(Pseudomonas syringae pv. actinidiae,Psa)作为指标菌筛选拮抗菌;结合菌落形态特征、生理生化特征及16S rRNA基因序列对拮抗菌进行鉴定;开展盆栽防治实验研究其对猕猴桃溃疡病的防治效率,并应用平板对峙法研究其对多种植物病原真菌的拮抗作用。结果共得到7株对Psa具有拮抗活性的内生细菌,其中,以菌株MA23的拮抗活性最为显著,鉴定为蜡样芽孢杆菌。MA23发酵液上清对猕猴桃溃疡病的盆栽防治效率为93.6%,同时其对多种病原真菌也具有拮抗活性。从银杏中筛选到一株对Psa具有较好拮抗活性的内生蜡样芽孢杆菌MA23,其对多种植物病原真菌也具有拮抗活性。

关键词: 内生细菌, 猕猴桃溃疡病, 蜡样芽孢杆菌, 抗真菌活性

Abstract:

The objective is to screen and identify the endophytic bacteria from wild Ginkgo biloba against the pathogen of kiwifruit canker,and to determine the bacteriostatic spectrum of common plant fungal diseases. Three-step method was used to isolate the endophytic bacteria from G. blioba. And then the Pseudomonas syringae pv.Actinidiae(Psa)was used as indicator bacterium for screening the antagonistic bacteria. Combination of morphological,physiological and biochemical characteristics and 16S rRNA sequence analysis was used to identify the antagonistic bacteria. Finally,the pot experiment was carried out to study its control efficacy against kiwifruit canker,and the confrontation method was used to evaluate the antifungal activity of the strain. Total 7 endophytic bacterial strains with antagonistic activities to Psa were obtained. The strain MA23 demonstrated the greatest antagonistic activity toward Psa and was identified as Bacillus cereus. The control efficacy of MA23 fermentation broth supernatant against kiwifruit canker was 93.6%. Meanwhile,MA23 had great capability of antagonism to some plant pathogenic fungi. In conclusion,endophytic bacterium B. cereus MA23 isolated from wild G. biloba not only has good antagonistic activity against the pathogen of kiwifruit canker,but also has significant inhibitory effect on a variety of plant pathogenic fungi.

Key words: endophytic bacteria, kiwifruit canker, Bacillus cereus, antifungal activity