泰山黄精内生细菌的抗菌活性研究

doi:10.13560/j.cnki.biotech.bull.1985.2020-0184

摘要/Abstract

摘要：

分离及筛选泰山黄精中对常见植物病原菌有拮抗活性的内生细菌,为发掘生防微生物资源及泰山黄精的开发利用奠定基础。用常规组织分离法从健康黄精根茎中进行菌种分离,平板对峙法对菌株进行筛选,依据形态、生理生化指标及16S rDNA序列测定对活性最强的菌株进行鉴定。采用平板倒扣法测定菌株产生挥发性物质的抑菌效果,通过琼脂扩散法测定无细胞发酵液对病原真菌的抑制活性。获得1株具有广谱抗菌活性的内生细菌HJ-12,经鉴定为短短芽孢杆菌（Brevibacillus brevis）,对6种病原菌的抑制率为（18.57±0.25）%-（48.33±0.15）%。该菌株所产的挥发性物质抑菌活性相较于菌体更强,抑制率为（21.05±0.17）%-（83.53±0.19）%,且该菌株经液体培养2 d后,其无细胞发酵液上清也具有明显的抑菌活性。该Brevibacillus brevis HJ-12菌株在植物真菌病害生物防控中具有潜在的应用价值。

关键词: 泰山黄精, 内生细菌, 植物病原菌, 抑菌活性

Abstract:

This work aims to isolate and screen endophytic bacteria of Polygonatum sibiricum Delar. ex Redoute with antifungal activity,and to lay foundation for exploring bio-control microbial resources and development and utilization of P. sibiricum Delar. ex Redoute. The bacterial strains were isolated from the roots and stems of healthy Polygonatum using conventional separation method,and then screened using the plate confrontation method. The strain with the best activity was identified based on phenotypical and biochemical properties as well as its 16S rDNA gene sequence. The antifungal effect of volatile compounds produced by the strain was measured by plate back-off method,and the inhibitory activity of cell-free fermentation broth to pathogen fungi was measured by agar diffusion method. An endophytic bacterium HJ-12 with broad-spectrum antifungal activity was obtained,and was identified as Brevibacillus brevis. The inhibition rate of HJ-12 to 6 phytopathogens ranged from（18.57±0.25）% to （48.33±0.15）%. The inhibition rate of the volatile compounds produced by HJ-12 was from （21.05±0.17）% to （83.53±0.19）%,and the cell-free fermentation liquid also demonstrated obvious antifungal activity after 2 d fermentation. In summary,the B. brevis HJ-12 presents potential application value in biological control of plant fungal diseases.

Key words: Polygonatum sibiricum Delar. ex Redoute, endophytic bacteria, phytopathogen, antifungal activity

郭晓平, 刘兴飞, 李晓楠, 吕雪茹, 郤少梅, 田园. 泰山黄精内生细菌的抗菌活性研究[J]. 生物技术通报, 2020, 36(11): 48-54.

GUO Xiao-ping, LIU Xing-fei, LI Xiao-nan, LÜ Xue-ru, XI Shao-mei, TIAN Yuan. Study on Antifungal Activity of an Endophytic Bacterium of Polygonatum sibiricum Delar. ex Redoute[J]. Biotechnology Bulletin, 2020, 36(11): 48-54.

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病原菌	抑菌率/%
白绢病菌	18.57±0.25
串珠镰刀菌	26.79±0.31
禾谷镰刀菌	19.23±0.21
尖孢镰刀菌	25.93±0.22
立枯丝核菌	48.33±0.15
层生镰刀菌	36.36±0.11

病原菌	抑菌率/%
白绢病菌	18.57±0.25
串珠镰刀菌	26.79±0.31
禾谷镰刀菌	19.23±0.21
尖孢镰刀菌	25.93±0.22
立枯丝核菌	48.33±0.15
层生镰刀菌	36.36±0.11

病原菌	抑菌率/%
白绢病菌	83.53±0.19
串珠镰刀菌	70.00±0.22
禾谷镰刀菌	61.43±0.36
尖孢镰刀菌	21.05±0.17
立枯丝核菌	76.67±0.18
层生镰刀菌	40.74±0.21

病原菌	抑菌率/%
白绢病菌	83.53±0.19
串珠镰刀菌	70.00±0.22
禾谷镰刀菌	61.43±0.36
尖孢镰刀菌	21.05±0.17
立枯丝核菌	76.67±0.18
层生镰刀菌	40.74±0.21