生物技术通报 ›› 2022, Vol. 38 ›› Issue (4): 242-252.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0964

• 研究报告 • 上一篇    下一篇

地黄不同时期内生促生细菌的筛选及其促生特性分析

王春艳1,2(), 腊贵晓3, 苏秀红1,2(), 李萌1,2, 董诚明1,2   

  1. 1.河南中医药大学,郑州 450046
    2.呼吸疾病中医药防治省部共建协同创新中心,郑州 450000
    3.河南省农业科学院经济作物研究所,郑州 450002
  • 收稿日期:2021-07-28 出版日期:2022-04-26 发布日期:2022-05-06
  • 通讯作者: 苏秀红,女,博士,教授,研究方向:中药资源学;E-mail: suxiuhong80@163.com
  • 作者简介:王春艳,女,硕士研究生,研究方向:中药资源学;E-mail: 1739080605@qq.com
  • 基金资助:
    国家重点研发计划资助项目(2017YFC1702800);河南省中药材产业技术体系(豫农科教【2018】14号)

Screening of Endophytic Bacteria from Rehmannia glutinosa at Different Stages and Analysis of Their Growth-promoting Characteristics

WANG Chun-yan1,2(), LA Gui-xiao3, SU Xiu-hong1,2(), LI Meng1,2, DONG Cheng-ming1,2   

  1. 1. Henan University of Chinese Medicine,Zhengzhou 450046
    2. Co-construction of Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases by Henan & Education Ministry of P.R. China,Zhengzhou 450000
    3. Industrial Crops Research Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002
  • Received:2021-07-28 Published:2022-04-26 Online:2022-05-06

摘要:

从地黄中开发优势菌种资源并分析促生特性,为开发地黄微生物专用促生菌肥提供基础。采用稀释平板法,以地黄不同时期根、茎、叶为材料分离内生细菌,根据形态学、生理生化、16S rRNA分析进行分类归属。选出54株内生细菌对产IAA、铁载体活性,溶磷以及固氮能力进行了评价,结果显示在不同时期共分离出512株内生细菌,将其归类为54组,每组选出1株进行检测,其中41株具有产IAA的能力;29株具有溶磷能力;28株具有产铁载体能力;25株具有固氮能力。该结果为研究地黄专用微生物促生菌肥提供参考,并为后续微生物促生菌肥应用到地黄大田实验中奠定基础。

关键词: 地黄, 分离鉴定, 内生细菌, 促生

Abstract:

The development of dominant strain resources from Rehmannia glutinosa and the analysis of growth promoting characteristics will provide a basis for the development of special growth-promoting bacterial fertilizer for R. glutinosa microorganisms. Dilution plate method was used to classify the endophytic bacteria isolated from the roots,stems and leaves of R. glutinosa at different stages to their morphologies,physiologies and biochemistries and 16S rRNA analysis. And the selected 54 strains of endophytic bacteria were evaluated based on their IAA-yielding capacities,iron-carrier activities,P-dissolving capacities,and N-fixing capacities. The results showed that 512 strains of endophytic bacteria were isolated in different periods and classified into 54 groups. One strain in each group was selected for detection,of which 41 strains had the ability to produce IAA;29 strains of them had the ability of dissolving phosphorus;28 strains had the ability to produce iron carrier;and 25 strains had nitrogen fixation ability. This result provides a reference for the study of special microbial growth-promoting bacteria fertilizer for R. glutinosa,and lays a foundation for the subsequent application of microbial growth-promoting bacteria fertilizer to the field experiment of R. glutinosa.

Key words: Rehmannia glutinosa., isolation and identification, endophytic bacteria, growth-promoting