生物技术通报 ›› 2022, Vol. 38 ›› Issue (7): 205-214.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0248

• 研究报告 • 上一篇    下一篇

荒漠草原柠条灌丛堆不同发育阶段土壤细菌群落结构特征

王子寅1,2,3(), 刘秉儒1,2,3(), 李子豪1,2,3, 赵晓玉1,2,3   

  1. 1.北方民族大学生物科学与工程学院,银川 750021
    2.国家民委黄河流域农牧交错区生态保护重点实验室,银川 750021
    3.宁夏特殊生境微生物资源开发与利用重点实验室,银川 750021
  • 收稿日期:2022-03-01 出版日期:2022-07-26 发布日期:2022-08-09
  • 作者简介:王子寅,男,硕士研究生,研究方向:植物与微生物生态学;E-mail: 846334252@qq.com
  • 基金资助:
    国家自然科学基金项目(31860158);宁夏科技创新领军人才项目(2021GKLRLX12);中央高校基本科研业务费北方民族大学高层次人才引进科研启动项目(2019KYQ001)

Characteristics of Soil Bacterial Community Structure in the Different Developmental Stages of Desert Grassland Caragana korshinskii Kom. Nebkhas

WANG Zi-yin1,2,3(), LIU Bing-ru1,2,3(), LI Zi-hao1,2,3, ZHAO Xiao-yu1,2,3   

  1. 1. College of Biological Science and Engineering,North Minzu University,Yinchuan 750021
    2. Key Laboratory of Ecological Protection of Agro-pastoral Ecotones in the Yellow River Basin,National Ethnic Affairs Commission of the People’s Republic of China,Yinchuan 750021
    3. Key Laboratory for the Development and Application of Microbial Resources in Extreme Environments,Yinchuan 750021
  • Received:2022-03-01 Published:2022-07-26 Online:2022-08-09

摘要:

为了解干旱半干旱地区荒漠草原不同发育阶段柠条灌丛堆土壤细菌群落结构与多样性特征及影响因素,选取宁夏荒漠草原生长期、成熟期和衰退期3种不同发育阶段柠条灌丛堆,通过高通量测序技术分析其土壤细菌的群落结构及多样性特征,探究发育阶段和土壤理化因子演变对土壤细菌群落结构和功能的影响因素,最后利用PICRUSt方法预测不同发育阶段柠条灌丛堆土壤细菌的功能。结果表明,获得的15 383个细菌操作分类单元(operational taxonomic unit,OTU)属于50门146纲364目590科1 111属,其中生长期细菌数量最多,优势菌门为放线菌门(Actinobacteriota)、变形菌门(Proteobacteria)、酸杆菌门(Acidobacteriota)、绿弯菌门(Chloroflexi)等9个菌门,占微生物总数90%以上,优势菌门门类与大多数研究相似。柠条灌丛堆不同发育阶段土壤AP、EC存在显著差异,表现为成熟期>生长期>衰退期,其他土壤养分差异不显著,但数值同样表现为成熟期>生长期>衰退期。细菌群落的多样性和丰富度没有显著性差异,但数值上表现为成熟期最高。AP、SOC是柠条灌丛堆土壤细菌群落多样性的主要影响因子,EC、pH等其他环境因子也显著影响多个菌群丰富度。基于柠条灌丛堆不同发育阶段土壤细菌基因组的16S rRNA序列对其功能的预测发现,土壤细菌的主要功能表现为代谢方面。以上结果丰富了半干旱荒漠草原地区土壤微生物多样性的相关理论知识,为柠条植被的恢复提供一定的依据。

关键词: 荒漠草原, 柠条, 灌丛堆, 土壤细菌, 群落结构, 功能预测

Abstract:

To understand the soil bacterial community structure and diversity characteristics and the influencing factors at different developmental stages of Caragana korshinskii Kom. nebkhas in arid and semi-arid desert grasslands,C. korshinskii Kom. nebkhas in Ningxia desert grasslands at three different developmental stages of growing,mature,and recession were selected. At the same time,high-throughput sequencing technology was applied to analyze their soil bacterial community structure and diversity characteristics,and to investigate the influencing factors of developmental stages and soil physicochemical factors evolution on soil bacterial community structure and function. Finally,PICRUSt method was to predict the functions of soil bacteria at different developmental stages of C. korshinskii Kom. nebkhas. The results showed that the obtained 15 383 operational taxonomic units(OTUs)belonged to 50 phyla,146 classes,364 orders,590 families,and 1 111 genera,with the largest number of bacteria at the growing stage and the dominant phyla being 9 of Actinobacteriota,Proteobacteria,Acidobacteriota,Chloroflexi,Crenarchaeota,and nine others. Proteobacteria,Acidobacteriota,Chloroflexi,and others,accounting for > 90% of the total number of microorganisms,and the dominant phyla were similar to most studies. There were significant differences in soil AP and EC in different development stages of C. korshinskii Kom. nebkhas,showing mature> growing > recession. Other soil nutrients were not significantly different,but the values were also expressed as mature> growing > recession. There was no significant difference in the diversity and abundance of bacterial communities,but the numerical value was the highest in the mature period. AP and SOC were the main factors influencing the diversity of soil bacterial communities in C. korshinskiiKom. nebkhas. Other environmental factors such as EC and pH also significantly affected the richness of multiple bacterial communities. Based on the prediction of 16S rRNA sequences of soil bacterial genomes in different developmental stages of C. korshinskii Kom. nebkhas,it was found that metabolism was the main function of soil bacteria. The above results can enrich the theoretical knowledge of soil microbial diversity in semi-arid desert grasslands and provide a reference for the restoration of C. korshinskii Kom. vegetation.

Key words: desert steppe, Caragana korshinskii Kom., nebkhas, soil bacteria, community structure, functional prediction