生物技术通报 ›› 2022, Vol. 38 ›› Issue (12): 244-251.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0363
收稿日期:
2022-03-28
出版日期:
2022-12-26
发布日期:
2022-12-29
作者简介:
高惠惠,女,硕士研究生,研究方向:微生物资源开发与利用;E-mail:基金资助:
GAO Hui-hui(), JIA Chen-bo, HAN Qin, SU Jian-yu, XU Chun-yan()
Received:
2022-03-28
Published:
2022-12-26
Online:
2022-12-29
摘要:
从根表微生物群落结构及病原菌两个角度探究宁杞7号枸杞根腐病发生的微生物学机制。基于高通量测序分析微生物群落组成及多样性;采用组织分离法获取腐根真菌,结合形态学与分子生物学特征鉴定真菌的分类,依据柯赫法则进行致病性验证。患病株根表真菌群落结构已严重失衡,不仅丰富度和多样性显著低于健康株,而且优势类群的变化很大。患病株根表的优势属为镰刀菌属和粘帚霉属,相对丰度分别为38.81%和31.24%;而健康株根表的优势属为被孢霉属(14.09%)和镰刀菌属(10.38%),患病株根表潜在的病原菌丰度显著高于健康株。从腐根样品中共分离到33株真菌,分属于镰刀菌属、青霉属、俄氏孔菌属和粘帚霉属4个属,其中分离频率最高的是镰刀菌属,达到 75.76%。镰刀菌属的2个种:腐皮镰刀菌(Fusarium solani)和尖孢镰刀菌(F. oxysporum)均可致宁杞7号枸杞患根腐病。本研究明确了宁杞7号枸杞根腐病的发生不仅与病原菌关系密切,而且与根表微生物的群落结构密切相关。
高惠惠, 贾晨波, 韩琴, 苏建宇, 徐春燕. 宁杞7号枸杞根腐病发生的微生物学机制[J]. 生物技术通报, 2022, 38(12): 244-251.
GAO Hui-hui, JIA Chen-bo, HAN Qin, SU Jian-yu, XU Chun-yan. Microbiological Mechanism of Root Rot of Lycium barbarum Ningqi-7[J]. Biotechnology Bulletin, 2022, 38(12): 244-251.
样品Sample | Sobs | Simpson | Shannon | ACE | Coverage |
---|---|---|---|---|---|
患病株Diseased | 195.00±35.12b | 0.32±0.18a | 1.88±0.60a | 340.94±39.89b | 99.9% |
健康株Healthy | 461.67±14.10a | 0.11±0.01a | 3.17±0.12a | 572.73±10.77a | 99.8% |
表1 根表真菌的Alpha多样性
Table 1 Alpha diversity of rhizoplane fungi of the healthy and diseased plants
样品Sample | Sobs | Simpson | Shannon | ACE | Coverage |
---|---|---|---|---|---|
患病株Diseased | 195.00±35.12b | 0.32±0.18a | 1.88±0.60a | 340.94±39.89b | 99.9% |
健康株Healthy | 461.67±14.10a | 0.11±0.01a | 3.17±0.12a | 572.73±10.77a | 99.8% |
图2 健康株和患病株门水平(A)和属水平(B)上根表真菌的组成与相对丰度
Fig. 2 Composition and relative abundance of rhizoplane fungi at the phylum(A)and genus(B)levels of healthy and diseased plants
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