生物技术通报 ›› 2022, Vol. 38 ›› Issue (12): 244-251.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0363

• 研究报告 • 上一篇    下一篇

宁杞7号枸杞根腐病发生的微生物学机制

高惠惠(), 贾晨波, 韩琴, 苏建宇, 徐春燕()   

  1. 宁夏大学生命科学学院 西部特色生物资源保护与利用教育部重点实验室,银川 750021
  • 收稿日期:2022-03-28 出版日期:2022-12-26 发布日期:2022-12-29
  • 作者简介:高惠惠,女,硕士研究生,研究方向:微生物资源开发与利用;E-mail:18700245852@139.com
  • 基金资助:
    宁夏自然科学基金项目(2020AAC02010)

Microbiological Mechanism of Root Rot of Lycium barbarum Ningqi-7

GAO Hui-hui(), JIA Chen-bo, HAN Qin, SU Jian-yu, XU Chun-yan()   

  1. School of Life Sciences,Ningxia University,Key Lab of Ministry of Education for Protection and Utilization of Special Biological Resources in the Western China,Yinchuan 750021
  • Received:2022-03-28 Published:2022-12-26 Online:2022-12-29

摘要:

从根表微生物群落结构及病原菌两个角度探究宁杞7号枸杞根腐病发生的微生物学机制。基于高通量测序分析微生物群落组成及多样性;采用组织分离法获取腐根真菌,结合形态学与分子生物学特征鉴定真菌的分类,依据柯赫法则进行致病性验证。患病株根表真菌群落结构已严重失衡,不仅丰富度和多样性显著低于健康株,而且优势类群的变化很大。患病株根表的优势属为镰刀菌属和粘帚霉属,相对丰度分别为38.81%和31.24%;而健康株根表的优势属为被孢霉属(14.09%)和镰刀菌属(10.38%),患病株根表潜在的病原菌丰度显著高于健康株。从腐根样品中共分离到33株真菌,分属于镰刀菌属、青霉属、俄氏孔菌属和粘帚霉属4个属,其中分离频率最高的是镰刀菌属,达到 75.76%。镰刀菌属的2个种:腐皮镰刀菌(Fusarium solani)和尖孢镰刀菌(F. oxysporum)均可致宁杞7号枸杞患根腐病。本研究明确了宁杞7号枸杞根腐病的发生不仅与病原菌关系密切,而且与根表微生物的群落结构密切相关。

关键词: 枸杞, 根腐病, 病原菌, 鉴定, 微生物群落结构

Abstract:

The purpose of this study is to explore the microbiological mechanism of the root rot of Lycium barbarum Ningqi-7 based on the analysis of microbial composition on rhizoplane and pathogenic microbes. High-throughput sequencing was used to analyze the microbial community composition and diversity. The root rot fungi were isolated with the tissue separation method. The combination of morphology and molecular characteristics was used to identify the fungi. The pathogenicity was identified according to Koch’s rule. The structure of fungal community on the rhizoplane of the diseased plant had been seriously imbalanced,with the richness and diversity being significantly lower than those of the healthy plants. The dominant groups of rhizoplane microorganisms were also quite different between the root rot diseased plants and the healthy plants. The dominant genera on the rhizoplane of the diseased plants were Fusarium and Clonostachys,with relative abundance being 38.81% and 31.24%,respectively;while the dominant genera on the rhizoplane of the healthy plants were Mortierella(14.09%)and Fusarium(10.38%). The abundance of potential pathogens on the rhizoplane of the diseased plants was significantly higher than that of the healthy plants. Total 33 fungi were isolated from the root rot of the diseased plants,and they were classified into four genera:Fusarium,Penicillium,Earliella,and Clonostachys,among which,the highest frequency of isolation was Fusarium,reaching 75.76%. Two species of Fusarium,F. solani and F. oxysporum,caused the root rot of Lycium barbarum Ningqi-7. This study clarified that the occurrence of root rot of L. barbarum Ningqi-7 was not only closely related to pathogenic fungi,but also related to the community structure of rhizoplane microorganisms.

Key words: Lycium barbarum, root rot, pathogenic microbe, identification, microbial community structure