链霉菌Streptomyces sp. FXP04全基因组测序分析

doi:10.13560/j.cnki.biotech.bull.1985.2023-0178

摘要/Abstract

摘要：

链霉菌Streptomyces sp. FXP04是健康马铃薯根际土壤中分离的一株对致病疫霉（Phytophthora infestans）有显著拮抗作用的革兰氏阳性放线菌。解析菌株FXP04的全部基因组序列信息，深入探索该菌株拮抗作用机制，并挖掘其次级代谢产物基因资源。利用Illumina和PacBio平台相结合测序技术对FXP04进行全基因组测序，并进行了基因预测、功能注释、比较基因组学分析以及次级代谢产物合成基因簇预测。FXP04基因组大小为4 535 201 bp，共编码5 037个基因，GC含量为72.95%，其中含有3个5S rRNA、3个16S rRNA、3个23S rRNA，33个tRNA以及23个sRNA；含有1 699个串联重复序列，含1 284个小卫星 DNA，188个微卫星 DNA；在 GO、COG、KEGG、CAZy、VFDB和ARDB数据库分别注释到2 088、2 319、1 530、73、139和14个基因；同时预测到菌株FXP04中有10个次级代谢产物合成基因簇，分别编码杀粉蝶菌素、Youssoufene等抑菌物质。通过基因组测序初步揭示了菌株FXP04的内在作用机制，对后续开发利用菌株FXP04提供理论依据。

关键词: 链霉菌, 基因组, 基因注释

Abstract:

Streptomyces sp. FXP04 is a gram-positive actinomycete isolated from the rhizosphere soil of healthy potato in Keshan county, Qiqihar, which has obvious antagonistic effect against Phytophthora infestans. This work aims to analyze the whole genome sequence information of strain FXP04, to explore the mechanism of action as well as the gene resources for the biosynthesis of secondary metabolites. The whole genome of Streptomycessp. FXP04 was sequenced by Illumina and PacBio platform, and gene prediction, functional annotation, comparative genomics analysis and synthesis gene cluster prediction of secondary metabolite were carried out. The genomic size of strain FXP04 was 4 535 201bp, encoding a total of 5 037 genes, with a GC content of 72.95%, including three 5S rRNA, three 16S rRNA, three 23S rRNA, 33 tRNA and 23 sRNA. It contained 1 699 tandem repeats，1 284 microsatellite DNA,188 microsatellite DNA. It was annotated to 2 088, 2 319, 1 530, 73, 139 and 14 genes in GO, COG, KEGG, CAZy, VFDB and ARDB databases, respectively. At the same time, it was predicted that there were 10 secondary metabolite synthesis gene clusters in strain FXP04, which encoded bacteriostatic substances such as piericidin, youssoufene and so on. The internal mechanism of strain FXP04 was analyzed by genome sequencing, which provides further theoretical basis for the development and utilization of strain FXP04.

Key words: Streptomyces, genome, gene annotation

唐碧瑶, 付学鹏. 链霉菌Streptomyces sp. FXP04全基因组测序分析[J]. 生物技术通报, 2023, 39(10): 268-280.

TANG Bi-yao FU Xue-peng. Sequencing Analysis of the Whole Genome of Streptomyces sp. FXP04[J]. Biotechnology Bulletin, 2023, 39(10): 268-280.

图/表 10

参考文献 40

[1]	Procópio RE, Silva IR, Martins MK, et al. Antibiotics produced by Streptomyces[J]. Braz J Infect Dis, 2012, 16(5): 466-471. doi: 10.1016/j.bjid.2012.08.014 pmid: 22975171
[2]	Dharmaraj S. Marine Streptomyces as a novel source of bioactive substances[J]. World J Microbiol Biotechnol, 2010, 26(12): 2123-2139. doi: 10.1007/s11274-010-0415-6 URL
[3]	Arasu MV, Duraipandiyan V, Ignacimuthu S. Antibacterial and antifungal activities of polyketide metabolite from marine Streptomyces sp. AP-123 and its cytotoxic effect[J]. Chemosphere, 2013, 90(2): 479-487. doi: 10.1016/j.chemosphere.2012.08.006 URL
[4]	Kim JD, Han JW, Lee SC, et al. Disease control effect of strevertenes produced by Streptomyces psammoticus against tomato Fusarium wilt[J]. J Agric Food Chem, 2011, 59(5): 1893-1899. doi: 10.1021/jf1038585 URL
[5]	胡磊, 牛世全, 景彩虹, 等. 拮抗油菜菌核病菌的链霉菌分离筛选与鉴定[J]. 中国油料作物学报, 2013, 35(1): 69-73. doi: 10.7505/j.issn.1007-9084.2013.01.012
	Hu L, Niu SQ, Jing CH, et al. Identification of Streptomyces antagonizing oilseed rape pathogen Sclerotinia sclerotiorum[J]. Chin J Oil Crop Sci, 2013, 35(1): 69-73.
[6]	Fu XP, Liu S, Ru JR, et al. Biological control of potato late blight by Streptomyces sp. FXP04 and potential role of secondary metabolites[J]. Biol Contr, 2022, 169: 104891. doi: 10.1016/j.biocontrol.2022.104891 URL
[7]	付学鹏, 沈童飞, 等. 链霉菌株Streptomyces sp.FXP04对水稻种子萌发和幼苗生长的影响[J]. 作物杂志, 2020(6): 163-169.
	Fu XP, Shen TF, et al. Effects of Streptomyces sp. FXP04 on seed germination and seedling growth of rice[J]. Crops, 2020(6): 163-169.
[8]	丁秀芹. 微生物基因组研究进展[J]. 科技资讯, 2009, 7(5): 11-12.
	Ding XQ. Research progress of microbial genome[J]. Sci Technol Inf, 2009, 7(5): 11-12.
[9]	高圣风, 徐毕爽, 等. 生防菌株Bacillus velezensis Z全基因组测序分析[J]. 热带作物学报, 2021, 42(5): 1216-1222.
	Gao SF, Xu BS, et al. Whole genome sequencing and analysis of the bio-control strain Bacillus velezensis Z[J]. Chin J Trop Crops, 2021, 42(5): 1216-1222.
[10]	Fu LM, Niu BF, Zhu ZW, et al. CD-HIT: accelerated for clustering the next-generation sequencing data[J]. Bioinformatics, 2012, 28(23): 3150-3152. doi: 10.1093/bioinformatics/bts565 pmid: 23060610
[11]	Nandi T, Ong C, et al. A genomic survey of positive selection in Burkholderia pseudomallei provides insights into the evolution of accidental virulence[J]. PLoS Pathog, 2010, 6(4): e1000845. doi: 10.1371/journal.ppat.1000845 URL
[12]	Edgar RC. MUSCLE: multiple sequence alignment with high accuracy and high throughput[J]. Nucleic Acids Res, 2004, 32(5): 1792-1797. doi: 10.1093/nar/gkh340 pmid: 15034147
[13]	Edgar RC. MUSCLE: a multiple sequence alignment method with reduced time and space complexity[J]. BMC Bioinformatics, 2004, 5: 113. pmid: 15318951
[14]	Blin K, Wolf T, et al. antiSMASH 4.0-improvements in chemistry prediction and gene cluster boundary identification[J]. Nucleic Acids Res, 2017, 45(W1): W36-W41. doi: 10.1093/nar/gkx319 URL
[15]	Lagesen K, Hallin P, Rødland EA, et al. RNAmmer: consistent and rapid annotation of ribosomal RNA genes[J]. Nucleic Acids Res, 2007, 35(9): 3100-3108. doi: 10.1093/nar/gkm160 pmid: 17452365
[16]	Lowe TM, Eddy SR. tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence[J]. Nucleic Acids Res, 1997, 25(5): 955-964. doi: 10.1093/nar/25.5.955 pmid: 9023104
[17]	Benson G. Tandem repeats finder: a program to analyze DNA sequences[J]. Nucleic Acids Res, 1999, 27(2): 573-580. doi: 10.1093/nar/27.2.573 pmid: 9862982
[18]	Zhou Y, Liang YJ, Lynch KH, et al. PHAST: a fast phage search tool[J]. Nucleic Acids Res, 2011, 39(Web Server issue): W347-W352. doi: 10.1093/nar/gkr485 URL
[19]	Luo YG, Lin SJ, et al. Regiospecific O-methylation of naphthoic acids catalyzed by NcsB1, an O-methyltransferase involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin[J]. J Biol Chem, 2008, 283(21): 14694-14702. doi: 10.1074/jbc.M802206200 pmid: 18387946
[20]	尹明明, 陈代杰, 等. Eremomycin的发酵条件优化及发酵产物的分离纯化[J]. 微生物学杂志, 2010, 30(4): 22-26.
	Yin MM, Chen DJ, et al. Fermentation conditions optimization, isolation and purification of eremomycin[J]. J Microbiol, 2010, 30(4): 22-26.
[21]	李永辉, 刘云, 王世春, 等. 大肠杆菌ppsA和tktA基因的串联表达[J]. 生物工程学报, 2003, 19(3): 301-306.
	Li YH, Liu Y, Wang SC, et al. Co-expressions of phosphoenolpyruvate synthetase a(ppsA)and transketolase A(tktA)genes of Escherichia coli[J]. Chin J Biotechnol, 2003, 19(3): 301-306.
[22]	Liu Z, Zhang YN, Sun JN, et al. A novel soluble squalene-hopene cyclase and its application in efficient synthesis of hopene[J]. Front Bioeng Biotechnol, 2020, 8: 426. doi: 10.3389/fbioe.2020.00426 URL
[23]	黄天培, 潘洁茹, 张鸿声, 等. 扁座壳孢Jos009胞外代谢产物15α, 22-二羟何帕烷的抗菌活性[J]. 应用与环境生物学报, 2013, 19(5): 878-880.
	Huang TP, Pan JR, Zhang HS, et al. Antimicrobial activity of 15α, 22-dihydroxyhopane produced by Aschersonia placenta Jos009[J]. Chin J Appl Environ Biol, 2013, 19(5): 878-880. doi: 10.3724/SP.J.1145.2013.00878 URL
[24]	Chen Z, Washio T, Sato M, et al. Cytotoxic effects of several hopanoids on mouse leukemia L1210 and P388 cells[J]. Biol Pharm Bull, 1995, 18(3): 421-423. pmid: 7550095
[25]	Dayanidhi DL, Somarelli JA, et al. Psymberin, a marine-derived natural product, induces cancer cell growth arrest and protein translation inhibition[J]. Front Med, 2022, 9: 999004. doi: 10.3389/fmed.2022.999004 URL
[26]	Li CS, Hu YF, Wu XH, et al. Discovery of unusual dimeric piperazyl cyclopeptides encoded by a Lentzea flaviverrucosa DSM 44664 biosynthetic supercluster[J]. Proc Natl Acad Sci USA, 2022, 119(17): e2117941119. doi: 10.1073/pnas.2117941119 URL
[27]	Patzer SI, Braun V. Gene cluster involved in the biosynthesis of griseobactin, a catechol-peptide siderophore of Streptomyces sp. ATCC 700974[J]. J Bacteriol, 2010, 192(2): 426-435. doi: 10.1128/JB.01250-09 pmid: 19915026
[28]	Matsuda K, Tomita T, Shin-Ya K, et al. Discovery of unprecedented hydrazine-forming machinery in bacteria[J]. J Am Chem Soc, 2018, 140(29): 9083-9086. doi: 10.1021/jacs.8b05354 pmid: 30001119
[29]	Kunze B, Höfle G, Reichenbach H. The aurachins, new quinoline antibiotics from myxobacteria: production, physico-chemical and biological properties[J]. J Antibiot, 1987, 40(3): 258-265. pmid: 3106289
[30]	Zhang M, Yang CL, et al. Aurachin SS, a new antibiotic from Streptomyces sp. NA04227[J]. J Antibiot, 2017, 70(7): 853-855. doi: 10.1038/ja.2017.50 pmid: 28420868
[31]	Pastor JM, Salvador M, Argandoña M, et al. Ectoines in cell stress protection: uses and biotechnological production[J]. Biotechnol Adv, 2010, 28(6): 782-801. doi: 10.1016/j.biotechadv.2010.06.005 pmid: 20600783
[32]	Shi J, Shi Y, Li JC, et al. In vitro reconstitution of cinnamoyl moiety reveals two distinct cyclases for benzene ring formation[J]. J Am Chem Soc, 2022, 144(17): 7939-7948. doi: 10.1021/jacs.2c02855 URL
[33]	Hall C, Wu M, Crane FL, et al. Piericidin A: a new inhibitor of mitochondrial electron transport[J]. Biochem Biophys Res Commun, 1966, 25(4): 373-377. doi: 10.1016/0006-291X(66)90214-2 URL
[34]	Zhou XF, Fenical W. The unique chemistry and biology of the piericidins[J]. J Antibiot, 2016, 69(8): 582-593. doi: 10.1038/ja.2016.71 pmid: 27301663
[35]	Darrouzet E, Issartel JP, Lunardi J, et al. The 49-kDa subunit of NADH-ubiquinone oxidoreductase(Complex I)is involved in the binding of piericidin and rotenone, two quinone-related inhibitors[J]. FEBS Lett, 1998, 431(1): 34-38. pmid: 9684860
[36]	Li WL, Zhang WY, Cheng YJ, et al. Investigation of carbonyl amidation and O-methylation during biosynthesis of the pharmacophore pyridyl of antitumor piericidins[J]. Synth Syst Biotechnol, 2022, 7(3): 880-886.
[37]	Zhou XF, Liang Z, Li KL, et al. Exploring the natural piericidins as anti-renal cell carcinoma agents targeting peroxiredoxin 1[J]. J Med Chem, 2019, 62(15): 7058-7069. doi: 10.1021/acs.jmedchem.9b00598 pmid: 31298537
[38]	Azad SM, Jin Y, Ser HL, et al. Biological insights into the piericidin family of microbial metabolites[J]. J Appl Microbiol, 2022, 132(2): 772-784. doi: 10.1111/jam.v132.2 URL
[39]	何亚文, Mazhari A., 崔莹, 等. 高产杀粉蝶菌素的链霉菌鉴定及其拮抗水稻白叶枯菌活性研究[J]. 微生物学报, 2023, 63(4): 1447-1459.
	He YW, Mazhari A, Cui Y, et al. A piericidin-producing Streptomyces strain: identification and antagonistic activity on Xanthomonas oryzae[J]. Acta Microbiol Sin, 2023, 63(4): 1447-1459.
[40]	卜庆廷, 毛旭明, 等. 链霉菌较小基因组研究进展及展望[J]. 生命科学, 2019, 31(4): 391-401.
	Bu QT, Mao XM, et al. Progress and prospect of genome-minimized Streptomyces hosts[J]. Chin Bull Life Sci, 2019, 31(4): 391-401.

Gene_ID	Align length/bp	Gene_ID	Align length/bp	Gene_ID	Align length/bp	Gene_ID	Align length/bp
FXP04GL000034	145	FXP04GL001071	217	FXP04GL002181	273	FXP04GL003852	312
FXP04GL000110	421	FXP04GL001084	256	FXP04GL002246	245	FXP04GL003853	236
FXP04GL000136	306	FXP04GL001105	240	FXP04GL002251	220	FXP04GL003854	216
FXP04GL000172	214	FXP04GL001156	979	FXP04GL002258	250	FXP04GL003869	162
FXP04GL000206	334	FXP04GL001158	209	FXP04GL002399	119	FXP04GL003888	175
FXP04GL000363	214	FXP04GL001159	549	FXP04GL002411	192	FXP04GL003982	283
FXP04GL000408	388	FXP04GL001160	407	FXP04GL002412	198	FXP04GL004032	236
FXP04GL000514	627	FXP04GL001161	260	FXP04GL002431	191	FXP04GL004050	405
FXP04GL000547	1999	FXP04GL001163	331	FXP04GL002686	60	FXP04GL004060	268
FXP04GL000548	1445	FXP04GL001165	239	FXP04GL002830	108	FXP04GL004064	172
FXP04GL000549	668	FXP04GL001238	257	FXP04GL002969	90	FXP04GL004069	217
FXP04GL000551	461	FXP04GL001239	376	FXP04GL002999	244	FXP04GL004082	230
FXP04GL000564	2121	FXP04GL001240	342	FXP04GL003031	402	FXP04GL004108	134
FXP04GL000565	1865	FXP04GL001265	205	FXP04GL003068	217	FXP04GL004109	181
FXP04GL000566	1515	FXP04GL001280	241	FXP04GL003104	222	FXP04GL004113	288
FXP04GL000569	565	FXP04GL001338	430	FXP04GL003123	89	FXP04GL004121	106
FXP04GL000605	240	FXP04GL001411	550	FXP04GL003154	149	FXP04GL004165	244
FXP04GL000609	708	FXP04GL001419	145	FXP04GL003179	228	FXP04GL004178	80
FXP04GL000639	403	FXP04GL001559	517	FXP04GL003222	426	FXP04GL004456	135
FXP04GL000646	217	FXP04GL001670	300	FXP04GL003249	287	FXP04GL004473	237
FXP04GL000652	234	FXP04GL001676	232	FXP04GL003255	338	FXP04GL004474	240
FXP04GL000653	501	FXP04GL001677	307	FXP04GL003405	240	FXP04GL004485	94
FXP04GL000663	306	FXP04GL001711	222	FXP04GL003459	185	FXP04GL004486	135
FXP04GL000684	110	FXP04GL001715	368	FXP04GL003460	134	FXP04GL004571	128
FXP04GL000722	73	FXP04GL001782	476	FXP04GL003505	226	FXP04GL004641	415
FXP04GL000785	212	FXP04GL001792	135	FXP04GL003545	98	FXP04GL004644	70
FXP04GL000792	334	FXP04GL001793	300	FXP04GL003552	92	FXP04GL004796	110
FXP04GL000926	200	FXP04GL001812	100	FXP04GL003574	213	FXP04GL004808	237
FXP04GL000927	443	FXP04GL001849	274	FXP04GL003661	171	FXP04GL004815	141
FXP04GL000969	219	FXP04GL001901	260	FXP04GL003662	273	FXP04GL004866	250
FXP04GL000972	249	FXP04GL001938	129	FXP04GL003663	259	FXP04GL004870	90
FXP04GL000973	503	FXP04GL001947	163	FXP04GL003664	282	FXP04GL004871	103
FXP04GL001037	306	FXP04GL001972	130	FXP04GL003701	41	FXP04GL004959	324
FXP04GL001053	162	FXP04GL002050	218	FXP04GL003794	393
FXP04GL001062	237	FXP04GL002068	268	FXP04GL003807	166

Gene_ID	Align length/bp	Gene_ID	Align length/bp	Gene_ID	Align length/bp	Gene_ID	Align length/bp
FXP04GL000034	145	FXP04GL001071	217	FXP04GL002181	273	FXP04GL003852	312
FXP04GL000110	421	FXP04GL001084	256	FXP04GL002246	245	FXP04GL003853	236
FXP04GL000136	306	FXP04GL001105	240	FXP04GL002251	220	FXP04GL003854	216
FXP04GL000172	214	FXP04GL001156	979	FXP04GL002258	250	FXP04GL003869	162
FXP04GL000206	334	FXP04GL001158	209	FXP04GL002399	119	FXP04GL003888	175
FXP04GL000363	214	FXP04GL001159	549	FXP04GL002411	192	FXP04GL003982	283
FXP04GL000408	388	FXP04GL001160	407	FXP04GL002412	198	FXP04GL004032	236
FXP04GL000514	627	FXP04GL001161	260	FXP04GL002431	191	FXP04GL004050	405
FXP04GL000547	1999	FXP04GL001163	331	FXP04GL002686	60	FXP04GL004060	268
FXP04GL000548	1445	FXP04GL001165	239	FXP04GL002830	108	FXP04GL004064	172
FXP04GL000549	668	FXP04GL001238	257	FXP04GL002969	90	FXP04GL004069	217
FXP04GL000551	461	FXP04GL001239	376	FXP04GL002999	244	FXP04GL004082	230
FXP04GL000564	2121	FXP04GL001240	342	FXP04GL003031	402	FXP04GL004108	134
FXP04GL000565	1865	FXP04GL001265	205	FXP04GL003068	217	FXP04GL004109	181
FXP04GL000566	1515	FXP04GL001280	241	FXP04GL003104	222	FXP04GL004113	288
FXP04GL000569	565	FXP04GL001338	430	FXP04GL003123	89	FXP04GL004121	106
FXP04GL000605	240	FXP04GL001411	550	FXP04GL003154	149	FXP04GL004165	244
FXP04GL000609	708	FXP04GL001419	145	FXP04GL003179	228	FXP04GL004178	80
FXP04GL000639	403	FXP04GL001559	517	FXP04GL003222	426	FXP04GL004456	135
FXP04GL000646	217	FXP04GL001670	300	FXP04GL003249	287	FXP04GL004473	237
FXP04GL000652	234	FXP04GL001676	232	FXP04GL003255	338	FXP04GL004474	240
FXP04GL000653	501	FXP04GL001677	307	FXP04GL003405	240	FXP04GL004485	94
FXP04GL000663	306	FXP04GL001711	222	FXP04GL003459	185	FXP04GL004486	135
FXP04GL000684	110	FXP04GL001715	368	FXP04GL003460	134	FXP04GL004571	128
FXP04GL000722	73	FXP04GL001782	476	FXP04GL003505	226	FXP04GL004641	415
FXP04GL000785	212	FXP04GL001792	135	FXP04GL003545	98	FXP04GL004644	70
FXP04GL000792	334	FXP04GL001793	300	FXP04GL003552	92	FXP04GL004796	110
FXP04GL000926	200	FXP04GL001812	100	FXP04GL003574	213	FXP04GL004808	237
FXP04GL000927	443	FXP04GL001849	274	FXP04GL003661	171	FXP04GL004815	141
FXP04GL000969	219	FXP04GL001901	260	FXP04GL003662	273	FXP04GL004866	250
FXP04GL000972	249	FXP04GL001938	129	FXP04GL003663	259	FXP04GL004870	90
FXP04GL000973	503	FXP04GL001947	163	FXP04GL003664	282	FXP04GL004871	103
FXP04GL001037	306	FXP04GL001972	130	FXP04GL003701	41	FXP04GL004959	324
FXP04GL001053	162	FXP04GL002050	218	FXP04GL003794	393
FXP04GL001062	237	FXP04GL002068	268	FXP04GL003807	166

Gene_ID	Subject ID	Resistance type	Resistance requirement	Antibiotic resistance
FXP04GL000228	ardb_195	pur8	--	Puromycin
FXP04GL000655	ardb_2322	bcra	bcrc	Bacitracin
FXP04GL001363	ardb_1271	emre	emrd	Aminoglycoside
FXP04GL001966	ardb_195	pur8	--	Puromycin
FXP04GL002045	ardb_2828	tcma	--	Tetracenomycin_c
FXP04GL002950	ardb_640	ykkd	ykkc	Na_antimicrobials
FXP04GL003556	ardb_1951	cml_e6	--	Chloramphenicol
FXP04GL003691	ardb_1951	cml_e6	--	Chloramphenicol
FXP04GL004392	ardb_2612	aph6ib	--	Streptomycin
FXP04GL004405	ardb_195	pur8	--	Puromycin
FXP04GL004638	ardb_195	pur8	--	Puromycin
FXP04GL004787	ardb_1043	vanha	vana,vanxa,vanya, vanra,vansa	Vancomycin, Teicoplanin
FXP04GL004788	ardb_2982	vand	vanhd,vanyd,vanxd,vanrd,vansd	Vancomycin, Teicoplanin
FXP04GL004789	ardb_993	vanxd	vand,vanhd,vanyd,vanrd,vansd	Vancomycin, Teicoplanin

Gene_ID	Subject ID	Resistance type	Resistance requirement	Antibiotic resistance
FXP04GL000228	ardb_195	pur8	--	Puromycin
FXP04GL000655	ardb_2322	bcra	bcrc	Bacitracin
FXP04GL001363	ardb_1271	emre	emrd	Aminoglycoside
FXP04GL001966	ardb_195	pur8	--	Puromycin
FXP04GL002045	ardb_2828	tcma	--	Tetracenomycin_c
FXP04GL002950	ardb_640	ykkd	ykkc	Na_antimicrobials
FXP04GL003556	ardb_1951	cml_e6	--	Chloramphenicol
FXP04GL003691	ardb_1951	cml_e6	--	Chloramphenicol
FXP04GL004392	ardb_2612	aph6ib	--	Streptomycin
FXP04GL004405	ardb_195	pur8	--	Puromycin
FXP04GL004638	ardb_195	pur8	--	Puromycin
FXP04GL004787	ardb_1043	vanha	vana,vanxa,vanya, vanra,vansa	Vancomycin, Teicoplanin
FXP04GL004788	ardb_2982	vand	vanhd,vanyd,vanxd,vanrd,vansd	Vancomycin, Teicoplanin
FXP04GL004789	ardb_993	vanxd	vand,vanhd,vanyd,vanrd,vansd	Vancomycin, Teicoplanin

簇编号 Cluster ID	基因起始-终止位置 Start-stop position of gene	基因数目 Number of genes	簇类型 Cluster type	已知基因簇 Known cluster	相似度 Similarity/%	来源 Resources
Cluster 1	94744-113290	15	Terpene	Petrichorin A /Petrichorin B	24	Lentzea flaviverrucosa
Cluster 2	293232-340393	27	NRPS	-	-	-
Cluster 3	570305-665318	54	T1PKS	Piericidin A1	100	Candidatus Streptomyces philanthi bv. triangulum
Cluster 4	666624-764035	47	T1PKS, transAT-PKS, PKS-like	Psymberin /Irciniastatin B	31	Uncultured bacterium psy1
Cluster 5	897552-922003	23	Terpene	Hopene	61	Streptomyces coelicolor A3（2）
Cluster 6	1024430-1065437	31	NRPS, NAPAA	Stenothricin	13	Streptomyces filamentosus NRRL 15998
Cluster 7	1268236-1317622	37	NRPS, NRP-metallophore	Griseobactin	53	Streptomyces sp. ATCC 700974
Cluster 8	1475651-1486055	9	Ectoine	Ectoine	100	Streptomyces sp. strain ID38640
Cluster 9	1945067-1985405	46	T3PKS	s56-p1	11	Streptomyces sp. SoC090715LN-17
Cluster 10	2502623-2524096	24	Lassopeptide	-	-	-
Cluster 11	2586833-2624623	45	Arylpolyene	Aurachin C/Aurachin D/Aurachin SS	13	Streptomyces sp.
Cluster 12	3558109-3579044	29	-	-	-	-
Cluster 13	3811714-3852580	49	Arylpolyene	Youssoufene A1/B1/B2/B3/B4	88	Streptomyces youssoufiensis