猕猴桃BBX基因家族成员鉴定与转录特征分析

doi:10.13560/j.cnki.biotech.bull.1985.2023-0812

摘要/Abstract

摘要：

【目的】BBX（B-Box）作为锌指转录因子亚家族之一，广泛参与植物的生长发育过程。本研究旨在揭示猕猴桃BBX家族的基因特征和潜在功能。【方法】从全基因组水平对猕猴桃BBX基因家族进行鉴定，分析其蛋白质理化性质、亚细胞定位、系统发育特征、蛋白质保守结构域以及同源特性等，同时研究它们在不同组织部位和果实贮藏过程的表达模式。【结果】48个AcBBX不均匀地分布于22条染色体上，编码的氨基酸长度126-515 aa，蛋白分子量14 172.09-57 905.84 Da，预测有4种亚细胞定位；家族成员有0-4个内含子；启动子含有光反应、植物激素调控、逆境胁迫调控、生长发育调控等多种顺式作用元件。结合进化树及保守结构域被聚为5个组，基因串联重复和片段复制是家族成员扩张的主要原因。AcBBXs在猕猴桃不同器官中的表达量有差异，7个成员在成熟果中表达量较其他部位高，3个成员在幼果中表达较高，7个成员在叶中表达量较高，3个成员在花中表达量较高；果实采后贮藏过程中，3个成员受果实外源激素调控影响表达上调且高于其他处理组，6个成员在果实室温贮藏一周后表达上调且高于其他处理组。在果实4℃低温贮藏过程中，10个成员的表达随时间增加而上调，5个成员的表达随时间增加而下调。【结论】揭示了BBX家族不同成员在猕猴桃生长发育和果实采后贮藏期间的转录特征，为后续猕猴桃BBX基因功能验证奠定基础。

关键词: 猕猴桃, BBX 转录因子, 全基因组鉴定, 不同组织部位, 果实贮藏, 转录分析

Abstract:

【Objective】 BBX（B-Box）, as one of the subfamilies of zinc finger transcription factors, is widely involved in the plant growth and development process. To reveal the genetic characteristics and potential functions of the kiwifruit BBX family.【Method】 The kiwifruit BBX gene family was identified at the whole genome level. And the protein physicochemical properties, subcellular localizations, systemic developmental characteristics, conserved protein domains, and homologous characteristics were analyzed. Meanwhile, the expression patterns in different tissues and different storage treatments were analyzed.【Result】 A total of 48 AcBBX genes were unevenly distributed across 22 chromosomes. The amino acid length of AcBBXs was 126-515 aa, protein molecular weight was 14 172.09-57 905.84 Da, and four subcellular localizations were predicted. There were 0 to 4 introns in AcBBX family. AcBBX promoters contained various cis-acting elements involved in light responsiveness, plant hormone regulatory, biotic and abiotic stress regulatory, growth and development regulatory. Combined the evolutionary tree with conserved domains, they could be clustered into five groups, and gene tandem duplication and fragment replication were the main reasons for the expansion of family members. The expressions of AcBBXs varied in different organs of kiwifruit. Seven AcBBX members had higher expressions in the mature fruits compared to other parts, 3 members having higher expressions in the young fruits, 7 members having higher expressions in the leaves, and 3 members having higher expressions in the flowers. During the post-harvest storage of fruits, 3 members were upregulated by exogenous hormones and higher than other processing groups, while 6 members were upregulated after one week of storage at room temperature and higher than other processing groups. During 4℃ storage of fruits, 10 members were upregulated, while 5 members were downregulated.【Conclusion】 This study reveals the transcriptional characteristics of different members of the kiwifruit AcBBX family during its growth, development, and post-harvest storages, laying the foundation for subsequent functional verification of the AcBBX gene.

Key words: kiwifruit, BBX transcription factor, whole genome identification, different tissues, fruit storage, transcription analysis

路喻丹, 刘晓驰, 冯新, 陈桂信, 陈义挺. 猕猴桃BBX基因家族成员鉴定与转录特征分析[J]. 生物技术通报, 2024, 40(2): 172-182.

LU Yu-dan, LIU Xiao-chi, FENG Xin, CHEN Gui-xin, CHEN Yi-ting. Identification of the Kiwifruit BBX Gene Family and Analysis of Their Transcriptional Characteristics[J]. Biotechnology Bulletin, 2024, 40(2): 172-182.

图/表 6

图1 猕猴桃与拟南芥BBX基因的系统进化树 I-V：AcBBX的5个组。红色字体：猕猴桃AcBBXs，黑色字体：拟南芥AtBBXs

Fig. 1 Phylogenetic analysis of the BBX genes between kiwifruit and Arabidopsis I-V: Five groups of AcBBX. Kiwifruit AcBBXs are in red, Arabidopsis AtBBXs are in black

图2 AcBBX基因家族的motif（A）、基因保守域（B）及基因结构（C）

Fig. 2 Motif（A）, conserved domains（B）and gene structures（C）of AcBBX gene family

图3 AcBBX基因的顺势作用元件分析

Fig. 3 Analysis of cis-acting elements in AcBBX genes

图4 AcBBX基因的种内共线性、染色体密度及染色体定位 ①：基因染色体定位；②：基因染色体密度热图；③：基因染色体密度线形图；④：种内共线性图

Fig. 4 Intraspecific collinearity of AcBBX genes and their chromosome density and locations ①: Gene chromosome locations. ②: Gene chromosome density heatmap. ③: Gene chromosome density line map. ④: Intra-species collinearity map

图5 AcBBX基因在猕猴桃不同组织部位的表达模式

Fig. 5 Expression patterns of the AcBBX genes in different tissues of kiwifruit

图6 AcBBX基因在猕猴桃果实不同贮藏处理下的表达模式 CK：成熟果（花后160 d）；RT_1W：成熟果室温25℃放置1周；ABA_1W：成熟果经50 mg/L的ABA浸泡2 min后置于室温25℃放置1周；Four_1W：成熟果在4℃放置1周；Four_2W：成熟果在4℃放置2周；Four_3W：成熟果在4℃放置3周

Fig. 6 Expression patterns of the AcBBX genes under different storage treatments CK: Mature fruits（160 d post flowering）; RT_1W: mature fruits stored at 25℃ for 1 week; ABA_1W: mature fruits soaked with 50 mg/L ABA for 2 min and then stored at 25℃ for 1 weeks; Four_1W: mature fruits stored at 4℃ for 1 week; Four_2W: mature fruits stored at 4℃ for 2 weeks; Four_3W: mature fruits stored at 4℃ for 3 weeks

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