水稻转导外源总DNA 变异后代盐胁迫下株高及生理指标分析

生物技术通报 ›› 2012, Vol. 0 ›› Issue (12): 71-75.

水稻转导外源总DNA 变异后代盐胁迫下株高及生理指标分析

肇莹¹, 王丽萍², 肖军¹, 王娜¹, 龚娜¹, 陈珣, ¹, 杨镇¹, 王红¹, 杨涛¹

1 辽宁省农业科学院微生物工程中心，沈阳 110161 ；2 沈阳农业大学农学院，沈阳 110161

收稿日期:2012-05-17 修回日期:2013-01-25 出版日期:2012-12-26 发布日期:2013-02-06
作者简介:肇莹, 女, 助理研究员, 研究方向作物遗传学；E-mail:zhaoying_226@163.com

Plant Height and Physiological Index Analysis of Variation Offspring ofRice Transduction Exogenous General DNA

Zhao Ying¹, Wang Liping², Xiao Jun¹, Wang Na¹, Gong Na¹, Chen Xun¹, Yang Zhen¹, Wang Hong¹, Yang Tao¹

1Liaoning Academy of Agricultural Sciences，Institute of Biotechnology，Shenyang 110161 ；2Shenyang Agricultural University，Shenyang 110161

Received:2012-05-17 Revised:2013-01-25 Published:2012-12-26 Online:2013-02-06

摘要/Abstract

摘要： 通过田间筛选的G 系列水稻耐盐新品系生理生化指标和形态学指标的测定，进一步鉴定筛选的耐盐水稻的耐盐性。采用沙培法培养水稻幼苗，在水稻幼芽期和两叶一心期进行盐处理，设清水、0.3% NaCl、0.6% NaCl、0.9% NaCl 4 个浓度盐溶液浇灌植株。前者，待两叶一心期，测定丙二醛（MDA）含量、脯氨酸（Pro）含量、超氧化物歧化酶（SOD）活性和株高；后者，从浇盐水当天开始算起，分别测定第3 天和第6 天水稻的上述4 个指标。结果显示，G16、G20 这两个新品系在两个处理下都是随着盐浓度的增加，MDA 含量逐渐降低，但在同一浇灌条件下均低于对照G6 ；Pro 含量逐渐升高，但在同一浇灌条件下均高于对照G6 ；SOD 活性先增强后减弱，但在同一浇灌条件下均高于对照G6 ；随着盐浓度的增加株高逐渐减小，但在同一浇灌条件下均高于对照G6。通过MDA 含量、PRO 含量、SOD 活性和株高的显著变化，由此得出田间筛选出的水稻G16、G20 的耐盐性都强于对照G6，从生理角度和形态学角度证实了田间筛选耐盐水稻结果的正确性，也证实了丙二醛含量、脯氨酸含量、SOD 活性和株高可以做为水稻耐盐性鉴定的生理生化指标和形态学指标。

关键词: 水稻, 耐盐, 株高, 生理指标

Abstract: Through inspecting physiological indexes and plant height of G series salt-tolerant rice varieties of field screening, we canfurther appraisal salt resistance of new screening salt-tolerant rice varieties. We cultivate rice seedlings by sand culture method. The ricein growing period of seedling and were treated with different concentrations of NaCl（0.3%, 0.6%, 0.9%）and clear water. The former, wemeasured MDA content, Pro content, SOD activity and palnt height in the third leaf stage. The above 4 indicators were measured in third day andsixth day, which begin to run from the day of pouring salt water. With the salt concentration increasing in the two treatments, the MDA contentin H5、H6 were gradually decreased, however, in the same watering conditions are lower than the control G6 ；Pro content increased gradually,However, in the same watering conditions are higher than the control G6 ；SOD content increased first, and then decreased, however, in the samewatering conditions are higher than the control G6. There were significant change in MDA content, Pro content, SOD activity and palnt height.The study was obtained that G series salt-tolerant rice varieties of field screening were stronger than the control G6. The results indicate thevalidity of G series salt-tolerant rice varieties from physiological indexes to plant height. Experiments conducted that MDA content ；Pro content,SOD activity and palnt height can be used as physiological indexes and morphological indicators for Identification of Genetic Dissection of SaltTolerance.

肇莹, 王丽萍, 肖军, 王娜, 龚娜, 陈珣, , 杨镇, 王红, 杨涛. 水稻转导外源总DNA 变异后代盐胁迫下株高及生理指标分析[J]. 生物技术通报, 2012, 0(12): 71-75.

Zhao Ying, Wang Liping, Xiao Jun, Wang Na, Gong Na, Chen Xun, Yang Zhen, Wang Hong, Yang Tao. Plant Height and Physiological Index Analysis of Variation Offspring ofRice Transduction Exogenous General DNA[J]. Biotechnology Bulletin, 2012, 0(12): 71-75.

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