天山雪莲PsaH基因的克隆及序列分析

生物技术通报 ›› 2013, Vol. 0 ›› Issue (6): 75-79.

天山雪莲PsaH基因的克隆及序列分析

张林华¹, 邱红林¹, 刘超¹, 王爱英², 邓福军³, 祝建波²

(1. 石河子大学生命科学学院, 石河子 832003;2. 石河子大学农业生物技术重点实验室, 石河子 832003;3. 新疆农垦科学院, 石河子 832000)

收稿日期:2013-06-20 修回日期:2013-06-20 出版日期:2013-06-20 发布日期:2013-06-20
作者简介:张林华, 男, 硕士研究生, 研究方向: 植物基因工程;E-mail: zhanglh1024@126.com
基金资助:
国家自然基金资助项目(31160049)

Cloning and Sequence Analysis of PsaH Gene from Sasussured involucrata Kar. et Kir

Zhang Linhua¹ Qiu Honglin¹ Liu Chao¹ Wang Aiying² Deng Fujun³ Zhu Jianbo²

(1. College of Life Science of Shihezi University, Shihezi 832003;2. Key Laboratory of Agricultural Biotechnology of Shihezi University, Shihezi 832003;3. Xinjiang Academy of Agriculture and Reclamation Science, Shihezi 832000)

Received:2013-06-20 Revised:2013-06-20 Published:2013-06-20 Online:2013-06-20

摘要/Abstract

摘要： Ps I H(Subunit H of photosystem I) 是属于光合系统I亚族的分子量约10 kD的内在膜蛋白, 是真核生物特有的3个组成光合系统I的亚基之一, 在植物光合作用中起重要作用。从天山雪莲(Sasussured involucrata Kar. et Kir) 叶片全长cDNA文库中, 筛选克隆得到PsaH基因。序列分析表明, 该基因的开放阅读框为432 bp, 编码143个氨基酸, 包括由47个氨基酸组成的转运肽和96个氨基酸组成的成熟PS I H。预测该蛋白质相对分子量为15.04 kD, 理论等电点(Theoretical pI) 为9.81, 存在一个跨膜结构。序列比对表明, SikPsaH编码蛋白与蓖麻(Ricinus communis) 、毛果杨(Populus trichocarpa) PsaH基因编码的蛋白质具有83.3%的相似性。系统进化树显示, 天山雪莲与石蒜(Lycoris radiata) 亲缘关系最近。。

关键词: 天山雪莲, PsaH基因, 克隆, 序列分析

Abstract: Ps I H is an intrinsic membrane protein of 10 kD that is a subunit of photosystem I(Ps I), Ps I H is one of the three PSI subunits found only in eukaryotes, plays an important role in plant photosynthesis. A cDNA clone encoding a PS I H subunit of photosystem I was cloned from Sasussured involucrata Kar. et Kir leaf cDNA library. Sequence analysis indicated that the psaH cDNA contained an open reading frame of 432 nucleotides encoding a 143 amino acid which consisted of a 47 amino acid transit peptide and a 96 amino acid mature PS I H peptide. Forecasted the protein relative molecular weight is 15.04 kD, Theoretical pI was 9.81, contains a span membrane structure. Sequence alignment indicated that the deduced amino acid sequence of Sasussured involucrata Kar. et Kir showed high identity of 83.3% to those of PsaH from Ricinus communis and Populus trichocarpa. Phylogenetic analysis further showed that the SikPsaH gene has the nearest relationship with Lycoris radiate.

Key words: Sasussured involucrata, Kar. et, Kir PsaH gene, Cloning, Sequence analysis

张林华, 邱红林, 刘超, 王爱英, 邓福军, 祝建波. 天山雪莲PsaH基因的克隆及序列分析[J]. 生物技术通报, 2013, 0(6): 75-79.

Zhang Linhua, Qiu Honglin, Liu Chao, Wang Aiying, Deng Fujun, Zhu Jianbo. Cloning and Sequence Analysis of PsaH Gene from Sasussured involucrata Kar. et Kir[J]. Biotechnology Bulletin, 2013, 0(6): 75-79.

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