Box-Behnken响应面设计法优化黑木耳菌质多糖提取工艺

生物技术通报 ›› 2013, Vol. 0 ›› Issue (6): 188-193.

Box-Behnken响应面设计法优化黑木耳菌质多糖提取工艺

赵超^{1, 2}, 曾峰¹, 黄一帆^{2, 3}, 刘斌^{1, 2}

(1. 福建农林大学食品科学学院, 福州 350002;2. 国家菌草工程技术研究中心, 福州 350002;3. 福建农林大学动物科学学院, 福州 350002)

收稿日期:2013-06-20 修回日期:2013-06-20 出版日期:2013-06-20 发布日期:2013-06-20
作者简介:赵超, 男, 博士, 讲师, 研究方向: 分子生物学与食药用菌功能成分;E-mail: zhchao@live.cn
基金资助:
国家菌草工程技术研究中心开放基金项目(JCJJ13012), 福建省教育厅科技项目(JB11038、JB12055)

Optimum Technique of Extracting Mycelium Polysaccharide from Auricularia auricular Using Box-Behnken Design-Response Surface Methodology

Zhao Chao^{1, 2} Zeng Feng^{1, 2} Huang Yifan^{2, 3} Liu Bin^{1, 2}

(1. College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002;2. National Engineering Research Center of Juncao, Fuzhou 350002;3. College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002)

Received:2013-06-20 Revised:2013-06-20 Published:2013-06-20 Online:2013-06-20

摘要/Abstract

摘要： 为了优化黑木耳菌质多糖的提取工艺, 采用Box-Behnken设计方法, 研究水提温度、水提时间、液料比及其交互作用对黑木耳菌质多糖得率的影响。应用Design Expert软件和响应面分析相结合的方法, 模拟得到回归方程的预测模型和可信度, 分析得到最佳的提取条件为: 水提温度81.59℃, 水提时间81.40 min, 液料比36.42:1。黑木耳菌质多糖得率的预测值为7.92 mg/g, 采用上述最佳提取条件进行验证试验, 测得菌质多糖得率为7.79 mg/g。采用响应法优化得到的黑木耳菌质多糖提取条件参数准确可靠, 该研究为黑木耳菌质多糖的工业化生产提供了必要的技术支持。

关键词: 黑木耳, 菌质多糖, 提取工艺, Box-Behnken设计, 响应面分析

Abstract: To optimize the extraction technique for mycelium polysaccharide from A. auricular, the effects of extraction temperature, extraction time, the liquid to solid ratio and their interaction on extraction rate were studied by Box-Behnken design. The predictive model and reliability were developed by Design Expert software and response surface analysis(RSM). The optimal extraction conditions were achieved and listed as follows: extraction temperature 81.59℃, extraction time 81.40 min, liquid-to-solid ratio 36.42:. Under the optimum conditions, the extraction yield of mycelium polysaccharide from A. auricular reached 7.79 mg/g, while the predicted one was 7.92 mg/g. The results showed the suitability of RSM in optimizing the extraction of mycelium polysaccharide from A. auricular. The necessary technical support was provided by this experimental study for the industrial production of mycelium polysaccharide from A. auricular.

Key words: Auricularia, auricular, Mycelium polysaccharide, Extraction technique, Box-Behnken design, Response surface analysis

赵超, 曾峰, 黄一帆, 刘斌. Box-Behnken响应面设计法优化黑木耳菌质多糖提取工艺[J]. 生物技术通报, 2013, 0(6): 188-193.

Zhao Chao, Zeng Feng, Huang Yifan, Liu Bin. Optimum Technique of Extracting Mycelium Polysaccharide from Auricularia auricular Using Box-Behnken Design-Response Surface Methodology[J]. Biotechnology Bulletin, 2013, 0(6): 188-193.

参考文献

[1] 唐青涛, 余若黔, 陈福生.深层发酵黑木耳产孢外多糖的初步研究[J].食品科学, 2002, 23(7):46-49.
[2] 周国华, 于国萍.黑木耳多糖降血脂作用的研究[J].现代食品科技, 2004, 21(1):46-58.
[3] Fan LS, Zhang SH, Yu L, et al. Evaluation of antioxidant property and quality of breads containing Auricularia auricula polysaccharide flour [J]. Food Chem, 2006, 101:1158-1163.
[4] Wu Q, Tan Z, Liu H, et al. Chemical characterization of Auricularia auricula polysaccharides and its pharmacological effect on heart antioxidant enzyme activities and left ventricular function in aged mice [J]. Int J Biol Macromol, 2010, 46:284-288.
[5] 谢意珍, 周静文, 李崇.黑木耳液体发酵条件的优化及发酵物的降血脂功能[J].食用菌学报, 2008, 15(2):47-50.
[6] 杨新美.中国食用菌栽培学[M].北京:农业出版社, 1988.
[7] 陈艳秋, 周丽萍, 尹英敏.黑木耳子实体水溶性多糖提取工艺的研究[J].吉林农业大学学报, 2003, 25(4):470-472.
[8] 朱磊, 王振宇, 周芳.响应面法优化微波辅助提取黑木耳多糖工艺研究[J].中国食品学报, 2009, 9(2):53-60.
[9] 韩春然, 唐娟, 马永强.黑木耳多糖的酶法提取、纯化及性质研究[J]. 食品科学, 2007, 28(2):53-55.
[10] 徐秀忠, 宋广磊.黑木耳多糖提取及多糖冲剂制备工艺研究[J]. 食品科技, 2012, 37(1):186-189.
[11] 王淑霞, 李爱梅, 张俊杰, 等.响应面分析法优化龙眼核中多酚物质提取工艺[J].食品科学, 2011, 32(10):35-39.
[12] 王钦德, 杨坚. 食品试验设计与统计分析基础[M].北京:中国农业大学出版社, 2009:106-107.
[13] 杨润亚, 杨树德, 董洪新, 等.响应面法优化猪肚菇多糖的提取工艺[J]. 食品科学, 2011, 32(14):29-30.
[14] Guo X, Zou X, Sun M. Optimization of extraction process by response surface methodology and preliminary characterization of polysaccharides from Phellinus igniarius [J]. Carbohyd Polym, 2010, 80(2):344-349.
[15] Zhong K, Wang Q. Optimization of ultrasonic extraction of polysaccharides from dried longan pulp using response surface methodology [J]. Carbohyd Polym, 2010, 80(1):19-25