咪唑乙烟酸降解菌的分离鉴定及降解条件优化

doi:10.13560/j.cnki.biotech.bull.1985.2017-0076

生物技术通报 ›› 2017, Vol. 33 ›› Issue (10): 163-168.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0076

咪唑乙烟酸降解菌的分离鉴定及降解条件优化

吕翻洋¹, 许泽华², 毛晓洁¹, 孙建光¹

1. 中国农业科学院农业资源与农业区划研究所农业部农业微生物资源收集与保藏重点实验室，北京 100081
2. 宁夏农林科学院宁夏回族自治区银川市 750002

收稿日期:2017-02-13 出版日期:2017-10-29 发布日期:2017-10-29
作者简介:吕翻洋，女，硕士研究生，研究方向：环境微生物；E-mail：lvfanyang150@163.com
基金资助:
公益性行业（农业）科研专项（201203045）

Isolation and Identification of an Imazethapyr-degrading Bacterium，and Optimization of Biodegradation Conditions

Lü Fan-yang¹, XU Ze-hua², MAO Xiao-jie¹, SUN Jian-guang¹

1. Key Laboratory of Microbial Resources，Ministry of Agriculture / Institute of Agricultural Resources and Regional Planning，Chinese Academy of Agricultural Sciences，Beijing 100081
2. Germplasm Resources Institute，Ningxia Academy of Agriculture and forestry Sciences，Yinchuan 750002

Received:2017-02-13 Published:2017-10-29 Online:2017-10-29

摘要/Abstract

摘要： 分离、鉴定除草剂咪唑乙烟酸降解菌IM9603并优化其降解条件。通过形态学，生理生化反应，16S rDNA初步鉴定该菌株分类地位，利用Box-Behnken优化该菌株对咪唑乙烟酸的降解条件。结果显示，初步鉴定该菌株属于表短杆菌属，在条件（pH5.5，温度26.2℃，接菌量1 mL（OD₆₀₀=1））下降解能力最强，菌株IM9603在咪唑乙烟酸初始浓度为50 mg/L 的条件下在7 d内降解率可达93.08%。分离出的咪唑乙烟酸降解菌属于Brevibacterium epidermidis菌株，该菌株对咪唑乙烟酸具有较高的降解能力，在环境修复方面存在潜在的应用前景。

关键词: 咪唑乙烟酸, 生物降解, 短杆菌属, 响应面分析

Abstract: This work aims to isolate and identify the strain IM9603 degrading herbicide imazethapyr，and optimize the degradation condition. IM9603 was identified as Brevibacterium sp. based on morphological，physio-biochemical characteristics，and analysis of 16S rDNA. Response surface methodology Box-Behnken was applied to optimize the degradation conditions. As results，strain IM9603 degraded 93.08% of the initial 50 mg/L supply of imazethapyr under the optimal condition（pH5 and 26.2℃）within 7 days，which was its best degradability. The isolated imazethapyr-degrading strain was Brevibacterium epidermidis，and presented high degradability to imazethapyr，suggesting that is owns a great potential in the environmental rehabilitation caused by imazethapyr.

Key words: imazethapyr, biodegradation, Brevibacterium sp., response surface methodology

吕翻洋, 许泽华, 毛晓洁, 孙建光. 咪唑乙烟酸降解菌的分离鉴定及降解条件优化[J]. 生物技术通报, 2017, 33(10): 163-168.

Lü Fan-yang, XU Ze-hua, MAO Xiao-jie, SUN Jian-guang. Isolation and Identification of an Imazethapyr-degrading Bacterium，and Optimization of Biodegradation Conditions[J]. Biotechnology Bulletin, 2017, 33(10): 163-168.

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咪唑乙烟酸降解菌的分离鉴定及降解条件优化

Isolation and Identification of an Imazethapyr-degrading Bacterium，and Optimization of Biodegradation Conditions

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