菌藻混合体系去除Cr（VI）的条件优化及Cr（VI） 还原酶活性的测定

doi:10.13560/j.cnki.biotech.bull.1985.2019-0322

摘要/Abstract

摘要： 为了高效去除废水中的Cr（Ⅵ）,从天津某印染场印染废水中筛选出两株高效除Cr（Ⅵ）细菌,探究了菌藻混合体系去除Cr（Ⅵ）的效果及优化条件,并测定了Cr（Ⅵ）还原酶活性。基于16S rRNA序列比对进行菌种鉴定。通过Placket-Burman、最陡爬坡试验、Box-Behnken实验设计及响应面法分析各因素交互作用。此外,对两株细菌Cr（Ⅵ）还原酶进行了活性测定。两株细菌属于沙雷氏菌属（Serratia）和代尔夫特菌属（Delftia）。最终确定菌藻混合体系去除Cr（Ⅵ）的最优条件为：温度29.74℃,Cu²⁺27.65 mg/L,葡萄糖含量2.41%（W/V）,丙酮酸钠2%（W/V）,接菌量7%,pH7.0,NaCl浓度为0,优化后菌藻混合体系24 h对Cr（Ⅵ）去除率达到97.89%。细菌S-3和D-7的Cr（Ⅵ）还原酶的最适温度为30℃,菌株S-3的Cr（Ⅵ）还原酶的最适pH值为6.0,酶促反应动力学常数K_m=86.94 µmol/L,V_max=2.71 µmol/（L·min）。菌株D-7中Cr（Ⅵ）还原酶最适pH值为7.0,酶促反应动力学常数K_m=103.18 µmol/L,V_max=3.38 µmol/（L·min）。菌藻混合体系通过响应面法优化后不但能提高Cr（Ⅵ）的去除率,也能缩短处理时间,在含铬废水处理方面具有良好的应用前景。

关键词: 菌藻混合, 微生物去除, 六价铬, 响应面分析

Abstract: In order to efficiently remove Cr（VI）from industry wastewater,two strains of bacteria that remarkably reduced Cr（VI）were isolated from the wastewater in a dyeing factory of Tianjin. The effect of removing Cr（VI）by the mixture of bacteria-microalgae system and the optimized conditions were studied,as well as the activity of Cr（VI）reductase was determined. 16S rRNA gene sequence alignment was used to identify the bacterial isolates,and Placket-Burman,the Steepest Ascent,Box-Behnken experimental design and response surface methodology were conducted to analyze the interactions among each factor. In addition,the Cr（VI）reductase activities of two bacteria were determined. The result showed that two strains of Cr（VI）reducing bacteria belonged to the genus Serratia and the genus Delftia. The optimal conditions for removing Cr（VI）by the mixture of bacteria-microalgae were as follows：the temperature was 29.74℃,the Cu²⁺ concentration was 27.65 mg/L,the glucose content was 2.41%（W/V）,the sodium pyruvate content was 2%（W/V）,the inoculum was 7%,and the pH was 7.0,and no NaCl,respectively. Under the optimized conditions,the removal rate of Cr（VI）by the mixture of bacteria-microalgae reached 97.89% in 24 h. The optimal temperature of the Cr（VI）reductase for two strains S-3 and D-7 was 30℃. The optimal pH of the Cr（VI）reductase for the strain S-3 was 6.0,and the kinetic constant of the enzymatic reaction was K_m=86.94 μmol/L,V_max=2.71 µmol/（L·min）. The optimal pH of the Cr（VI）reductase for the strain D-7 was 7.0,and the kinetic constant of the enzymatic reaction was K_m=103.18 μmol/L,V_max=3.38 µmol/（L·min）. In conclusion,the optimized mixture of bacteria-microalgae may not only increase the removal rate of Cr（VI）,but also shorten the treatment time,and it has a promising application prospect in the treatment of chromium-containing wastewater.

Key words: mixture of bacteria-microalgae, microbial removal, Cr（Ⅵ）, response surface methodology

杨胜男, 刘娜, 宋东辉. 菌藻混合体系去除Cr（VI）的条件优化及Cr（VI）还原酶活性的测定[J]. 生物技术通报, 2019, 35(9): 83-92.

YANG Sheng-nan, LIU Na, SONG Dong-hui. Optimization of Chromium（VI）Removal by Mixture of Bacteria-microalgae and Determination of Chromium（VI）Reductase Activities[J]. Biotechnology Bulletin, 2019, 35(9): 83-92.

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