Principle of Whole Genome Amplification Technology and Its Progress

doi:10.13560/j.cnki.biotech.bull.1985.2014.12.008

Abstract

Abstract: The rapid developments of DNA array and next generation sequencing technologies had greatly promoted molecular biology techniques to be used in various fields. However, the limited quantity and quality of sample DNA may affect large scale genetic analyses. To overcome these problems, the whole genome amplification(WGA)technology was employed to amplify trace amounts of genomic DNA to sufficient quantity to meet the requirements of high throughput analyses. The highly efficient and reliable WGA methods also helped to realize large scale of genetic analyses based on even single cell. Moreover, as the single cell level research continues, more efficient WGA approaches were developed. This paper summarized the general WGA methods frequently used before and newly developed methods on basic principle level as well as the advantages and disadvantages of each method, which helped to make good use of the powerful WGA techniques.

Key words: Whole genome amplification, Single cell sequencing, Genotyping, Genome variations, Mutation detection

Pan Xiaoming, Liang Xingguo. Principle of Whole Genome Amplification Technology and Its Progress[J]. Biotechnology Bulletin, 2014, 0(12): 47-54.

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