Application and Prospect of KASP Marker Technology in Main Crops

doi:10.13560/j.cnki.biotech.bull.1985.2021-1378

Abstract

Abstract:

With the development of gene sequencing technology,plant genome data are becoming more and more abundant. Single nucleotide polymorphism（SNP）data are widely used in the development and application of molecular markers because of their high density,high throughput and easy automated analysis. Kompetitive allele-specific PCR（KASP）is a high-throughput genotyping technology mainly based on SNP. Because of its high flux,low cost and strong operability,this technology has great application potential in the field of crop character improvement. This paper introduces the development,principle and method steps of KASP technology,summarizes the application of KASP technology in genetic breeding such as germplasm resource identification,molecular marker assisted breeding,gene mapping and seed purity identification of main crops,and discusses the advantages and disadvantages of KASP technology in order to provide reference basis for crop breeding research in the future.

Key words: KASP, high-throughput genotyping, genetic identification, molecular breeding, improvement of agronomic characters

YANG Qing-qing, TANG Jia-qi, ZHANG Chang-quan, GAO Ji-ping, LIU Qiao-quan. Application and Prospect of KASP Marker Technology in Main Crops[J]. Biotechnology Bulletin, 2022, 38(4): 58-71.

Figures/Tables 2

References 101

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作物Crops	应用Application	参考文献 Reference
水稻 Oryza sativa	种质资源鉴定与亲缘关系研究	[18-20]
	分子标记辅助育种	[33-41]
	遗传图谱构建与基因定位	[67-68,70-71]
小麦 Triticum aestivum	种质资源鉴定与亲缘关系研究	[21-23]
	分子标记辅助育种	[42-49]
	遗传图谱构建与基因定位	[72-76]
玉米 Zea mays	种质资源鉴定与亲缘关系研究	[24]
	分子标记辅助育种	[50,53]
	遗传图谱构建与基因定位	[77,79-80]
	种子纯度鉴定	[91-93]
大白菜 Brassica rapa	种质资源鉴定与亲缘关系研究	[25]
	遗传图谱构建与基因定位	[85]
西兰花 Brassica oleracea	种质资源鉴定与亲缘关系研究	[26]
葡萄 Vitis vinifera	种质资源鉴定与亲缘关系研究	[27]
小扁豆 Lens culinaris	种质资源鉴定与亲缘关系研究	[28]
咖啡 Coffea arabica	种质资源鉴定与亲缘关系研究	[29]
花生 Arachis hypogaea	种质资源鉴定与亲缘关系研究	[30]
	遗传图谱构建与基因定位	[86]
豇豆 Vigna unguiculata	分子标记辅助育种	[54]
番茄 Lycopersicon esculentum	分子标记辅助育种	[55]
西瓜 Citrullus lanatus	分子标记辅助育种	[56]
高粱 Sorghum bicolor	分子标记辅助育种	[58]
	遗传图谱构建与基因定位	[81]
桃 Amygdalus persica	分子标记辅助育种	[59]
马铃薯 Solanum tuberosum	分子标记辅助育种	[60]
苹果 Malus pumila	分子标记辅助育种	[61]
	遗传图谱构建与基因定位	[83]
辣椒 Capsicum annuum	分子标记辅助育种	[62]
向日葵 Helianthus annuus	分子标记辅助育种	[63]
大豆 Glycine max	遗传图谱构建与基因定位	[82]
洋葱 Allium cepa	遗传图谱构建与基因定位	[84]
瓠瓜 Lagenaria siceraria	种子纯度鉴定	[94]
棉花 Gossypium hirsutum	种子纯度鉴定	[95]
大麦 Hordeum vulgare	种子纯度鉴定	[96]
萝卜 Raphanus sativus	种子纯度鉴定	[97]

作物Crops	应用Application	参考文献 Reference
水稻 Oryza sativa	种质资源鉴定与亲缘关系研究	[18-20]
	分子标记辅助育种	[33-41]
	遗传图谱构建与基因定位	[67-68,70-71]
小麦 Triticum aestivum	种质资源鉴定与亲缘关系研究	[21-23]
	分子标记辅助育种	[42-49]
	遗传图谱构建与基因定位	[72-76]
玉米 Zea mays	种质资源鉴定与亲缘关系研究	[24]
	分子标记辅助育种	[50,53]
	遗传图谱构建与基因定位	[77,79-80]
	种子纯度鉴定	[91-93]
大白菜 Brassica rapa	种质资源鉴定与亲缘关系研究	[25]
	遗传图谱构建与基因定位	[85]
西兰花 Brassica oleracea	种质资源鉴定与亲缘关系研究	[26]
葡萄 Vitis vinifera	种质资源鉴定与亲缘关系研究	[27]
小扁豆 Lens culinaris	种质资源鉴定与亲缘关系研究	[28]
咖啡 Coffea arabica	种质资源鉴定与亲缘关系研究	[29]
花生 Arachis hypogaea	种质资源鉴定与亲缘关系研究	[30]
	遗传图谱构建与基因定位	[86]
豇豆 Vigna unguiculata	分子标记辅助育种	[54]
番茄 Lycopersicon esculentum	分子标记辅助育种	[55]
西瓜 Citrullus lanatus	分子标记辅助育种	[56]
高粱 Sorghum bicolor	分子标记辅助育种	[58]
	遗传图谱构建与基因定位	[81]
桃 Amygdalus persica	分子标记辅助育种	[59]
马铃薯 Solanum tuberosum	分子标记辅助育种	[60]
苹果 Malus pumila	分子标记辅助育种	[61]
	遗传图谱构建与基因定位	[83]
辣椒 Capsicum annuum	分子标记辅助育种	[62]
向日葵 Helianthus annuus	分子标记辅助育种	[63]
大豆 Glycine max	遗传图谱构建与基因定位	[82]
洋葱 Allium cepa	遗传图谱构建与基因定位	[84]
瓠瓜 Lagenaria siceraria	种子纯度鉴定	[94]
棉花 Gossypium hirsutum	种子纯度鉴定	[95]
大麦 Hordeum vulgare	种子纯度鉴定	[96]
萝卜 Raphanus sativus	种子纯度鉴定	[97]