Biotechnology Bulletin ›› 2015, Vol. 31 ›› Issue (5): 106-112.doi: 10.13560/j.cnki.biotech.bull.1985.2015.05.017

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Cloning and Expression Analysis of Proline Transporter Gene in Ammopiptanthus mongolicus

Yue Guangzhen, Jin Man, Li Junlin, Yang Shunying, Guo Manyuan, Su Yanhua   

  1. (State Key Laboratory of Soil and Sustainable Agriculture/Institute of Soil Science,Chinese Academy of Sciences,Nanjing 210008)
  • Received:2014-09-11 Online:2015-05-18 Published:2015-05-18

Abstract: The cDNA of proline transporter gene in Ammopiptanthus mongolicus(GenBank accession No. KJ873133)was amplified by RACE technology. Bioinformatics analysis showed that the length of AmProT ORF was 1 329 bp, the gene encoded a putative polypeptide of 442 amino acids with a calculated molecular mass of 48.0733 kD and a theoretical pI of 9.32. It had 11 transmembrance domains, which is the typical characteristics of proline translocator. Phylogenic analysis indicated that AmProT was 86% similar to proline transporter gene of Glycine max. Real-time PCR analysis revealed that AmProT expressed in all tissues examined with more higher level in the above-ground part than the underground part; expression profiles under different stress treatments such as drought, salt and ABA were compared, and the results revealed that transcriptional level of AmProT was up-regulated. It is speculated that AmProT plays an important role in response to abiotic stresses such as drought and salt.

Key words: Ammopiptanthus mongolicus, proline transporter gene, gene cloning, expression analysis