Abstract: Bacitracin has been widely utilized in livestock breeding and cultivation, and it is necessary to screen strains for improving bacitracin production. In this study, low concentration of bacitracin was used to screen bacitracin producer, and one strain was isolated and identified as Bacillus licheninformis strain Y822. The optimal culture condition for the strain was pH7.0, and the improvement of oxygen supply was favorable to the production of bacitracin. B. licheninformis Y822 was sub-cultured for 5 generations. Each generation of the strain was used to produce bacitracin using the optimized fermentation condition. The average value of the produced bacitracin from 5 generations of B. licheninformis Y822 was （783.51±7.34）U/mL, and the percentage of bacitracin A was about（75.04±0.83）%. The production of bacitracin by B. licheniformis Y822 was stable and efficient. Therefore, B. licheniformis Y822 was a potential candidate for producing bacitracin in industry.

Key words: Bacillus licheninformis, bacitracin, isolation, identification