Cloning of CAV-3 in Yak and Analysis Its Expression in Yak and Cattle

doi:10.13560/j.cnki.biotech.bull.1985.2015.05.030

Abstract

Abstract: This study aims to clone, analyze bioinformatics and determine the expression pattern of CAV-3 gene in yak. A pair of special primers were designed according to released mRNA sequence of bovine CAV-3 in GenBank. A coding region sequence of yak CAV-3 was amplified by RT-PCR; the general physical and chemical properties, hydrophobicity, protein domains and protein secondary structures were systemically analyzed and predicted by bioinformatics techniques. The expression levels of CAV-3 mRNA in some organs of yak and cattle were detected by semi-quantitative PCR. Real-time PCR was employed to examine the expression levels of CAV-3 mRNA in yak and cattle muscles. The coding region sequence of CAV-3 gene in yak contains a complete ORF（631 bp）which encoded 151 amino acids. CAV-3 mRNA expression in the heart and muscle was detected, but not in any other examined tissues, and its expression level in the heart was higher than in the muscle. The result in yak was consistent with that in cattle. Expression of CAV-3 gene in the yak muscle was less than in the cattle muscle, but not significantly（P>0.05）. The cloning and analysis of CAV-3 provided scientific basis for further study the function of CAV-3 gene in muscle development and muscle physiological process in the future.

Key words: yak, Caveolin-3, cloning, expression analysis

Zhao Juanhua, Pei Jie, Liang Chunnian, Guo Xian, Wu Xiaoyun, Zhang Liangbin, Yan Ping. Cloning of CAV-3 in Yak and Analysis Its Expression in Yak and Cattle[J]. Biotechnology Bulletin, 2015, 31(5): 194-199.

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