Polymorphism Analysis of MSTN Gene Exons of Ganzhou Simmental Cattle in Gansu Province

doi:10.13560/j.cnki.biotech.bull.1985.2015.06.026

Biotechnology Bulletin ›› 2015, Vol. 31 ›› Issue (6): 165-169.doi: 10.13560/j.cnki.biotech.bull.1985.2015.06.026

• Report • Previous Articles Next Articles

Polymorphism Analysis of MSTN Gene Exons of Ganzhou Simmental Cattle in Gansu Province

Li Jiyou^1，4, Liang Chunhua², Liu Xia^1，3, Sun Xuejing², Du Xiaohua^1，2

1. Gansu Key Laboratory of Herbivorous Animal Biotechnology，Lanzhou 730070；2. College of Veterinary Medicine，Gansu Agricultural University，Lanzhou 730070；3. College of Life Science and Technology，Gansu Agricultural University，Lanzhou 730070；4 Gansu Provincial Bureau of Animal Husbandry Industry Management，Lanzhou 730030

Received:2014-10-29 Online:2015-06-19 Published:2015-06-20

Abstract

Abstract: PCR-SSCP(single-strand conformational polymorphism)was used to detect the genetic polymorphism in 3 exons of myostatin(MSTN)gene in 50 individuals of Ganzhou Simmental cattle in Gansu. The alleles in a population were verified by sequencing, which provided the theoretical evidences for investigating the correlation between MSTN gene of Simmental cattle and meat quality. The results showed that the polymorphism in the exon 2 was controlled by B and C alleles, which formed 3 genotypes of BB, CC and BC with the frequencies at 0. 22(11/50), 0. 64(32/50)and 0. 14(7/50)respectively. The polymorphism in the exon 1 and exon 3 was controlled by A and D allele respectively, which formed AA and DD genotypes with frequencies at 1(50/50). Sequence analysis indicated that there was one single nucleotide mutation of C to T at 41^st nucleotide in the exon 2 of Ganzhou Simmental cattle；however this did not lead to the amino acid changed, so it was a synonymous mutation. There were no mutations in the exon 1 and exon 3. The statistical results showed that the exon 2 of MSTN gene in 50 individuals of Ganzhou Simmental cattle had a moderate level of polymorphism, and the exon 1 and exon 3 had no polymorphism.

Key words: MSTN gene, exon, genetic polymorphism, Simmental cattle

Li Jiyou, Liang Chunhua, Liu Xia, Sun Xuejing, Du Xiaohua. Polymorphism Analysis of MSTN Gene Exons of Ganzhou Simmental Cattle in Gansu Province[J]. Biotechnology Bulletin, 2015, 31(6): 165-169.

References

[1] Lee SJ, McPherron AC. Myostatin and the control of skeletal muscle mass：Commentary[J]. Current Opinion in Genet ic & Development, 1999, 9(5)：604-607.
[2] McPherron AC. Lee SJ. Double muscling in cattle due to mutations in the myostatin gene[J]. Proc Natl Acad Sci USA, 1997, 94(23)：12457-12461.
[3]鲍淑青. 重组肌肉生长抑制素的制备及主动免疫生长猪的研究［D]. 雅安：四川农业大学, 2007.
[4]Liu D, Xu Q, Zang L, et al. Identification and genetic effect of haplotypes in the promoter region of porcine myostatin gene[J]. Animal Cenetics, 2011, 42(1)：6-14.
[5]刘晓琴, 马喜山, 唐中林, 等. 猪MSTN基因的多态性和生长性状关联分析[J]. 畜牧兽医学报, 2013, 44(7)：1063-1069.
[6]Hu S, Ni W, Sai W, et al. Sleeping Beauty-mediated knockdown of sheep myostatin by RNA interference[J]. Biotechnol Lett, 201l, 33(10)：1949-1953.
[7] 李帅, 杨舒黎, 苟潇, 等. 肌肉生成抑制素(MSTN)基因研究进展[J]. 中国畜牧兽医, 2011, 38(2)：60-63.
[8] 梁春年, 阎萍, 邢成峰, 等. 牦牛MSTN基因内含子2多态性及与生产性状的相关性[J]. 华中农业大学学报, 2011, 30(3)：285-289.
[9] 徐良梅, 李仲玉, 单安山. 肌肉生成抑制素(MSTN)基因及其在畜牧业上的应用[J]. 东北农业大学学报. 2008, 39(6)：141-144.
[10] Lee SJ, McPherron AC. Regulation of myostatin activity and muscle growth[J]. Proc Natl Acad Sci USA, 2001, 98(16)：9306-9311.
[11]Kambadur R, Sharma M, Smith TP, et al. Mutations in myostatin(GDF-8)in double-muscled Belgian Blue and Piedmontese cattle[J]. Genome Res, 1997, 7(9)：910-916.
[12] Miranda ME, Amigues Y, Boscher MY, et al. Simultaneous genoty-ping to detect myostatin gene polymorphism in beef cattle breeds[J]. Animal Breed Genet, 2002, 119(6)：361-366.
[13]Grobet L, Poncelet D, Royo LJ, et al. Molecular definition of an allelic series of mutations disrupting the myostatin function and causing double- muscling in cattle[J]. Mammalian Genome, 1998, 9(3)：210-213.
[14]冀德君, 常洪, 常春芳, 等. 中国牛属4个物种MSTN基因的遗传变异研究[J]. 畜牧兽医学报, 2008, 39(6)：701-704.
[15]吕文发, 杨连玉, 贺实伟, 等. 中国西门塔尔牛、利木赞牛和夏洛莱牛MSTN基因多态性分析[J]. 中国兽医学报, 2010, 30(6)：852-868.
[16]Botstein D, White RL, Skolnick M, et al. Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J]. American Journal of Human Genetics, 1980, 32(3)：314-331.

Polymorphism Analysis of MSTN Gene Exons of Ganzhou Simmental Cattle in Gansu Province

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 6

Recommended Articles

Metrics

Comments

[1]	LI Biao, ZHANG Rui-ying, WANG Xiao-qi, ZHANG Cun-fang, DUAN Zi-yuan. Microsatellite Polymorphism and Its Correlation Analysis with Body Size Traits of Tan Sheep [J]. Biotechnology Bulletin, 2019, 35(6): 131-137.
[2]	SONG Huan, LUO Yun-bo, XU Wen-tao. Research Progress on Exonuclease III-assisted Functional Nucleic Acid Biosensors [J]. Biotechnology Bulletin, 2018, 34(9): 55-69.
[3]	LEI Chen, LIU Sheng-li, YU Ting-qiao, CHEN Yu-zhen, LU Cun-fu. Bioinformatics Analysis of Genomic Structure and Introns of AmCIP Gene from Ammopiptanthus mongolicus [J]. Biotechnology Bulletin, 2016, 32(9): 93-99.
[4]	Liang Chunhua, Liu Xia, Sun Xuejing, Luo Yuzhu, Gao Xuejin, Du Xiaohua. Genetic Variation Study of NGB Gene Exon 3 of Simmental Cattle Hybrid Taxon in Gansu Hexi Regions [J]. Biotechnology Bulletin, 2015, 31(2): 122-128.
[5]	Zhang Guohua, Ma Xiaojun, Lü Weili,. Analyzing Polymorphism of SLA-DQA Gene Exon 2 in the Hezuopig [J]. Biotechnology Bulletin, 2014, 0(4): 91-95.
[6]	Niu Junqi,Wu Chaoxing,Yang Litao,Li Yangrui. Analysis of Genomic Structure and Introns of SoNIN1 Gene from Sugarcane [J]. Biotechnology Bulletin, 2014, 0(12): 147-152.