Analysis of Genomic Structure and Introns of SoNIN1 Gene from Sugarcane

doi:10.13560/j.cnki.biotech.bull.1985.2014.12.024

Abstract

Abstract: According to the full length cDNA and part of the promoter sequences of the SoNIN1 gene we designed primers，amplified the SoNIN1 gene form gDNA of sugarcane leaves by PCR, and analyzed the gene structure of SoNIN1 by using bioinformatics methods. The results showed that the DNA sequence of the SoNIN1 gene is 4 938 bp, containing six exons and five introns, GenBank accession number KF563902. 5’UTRs of the SoNIN1 gene contain 268 bp introns, and five introns contain low temperature, drought, and hormonal stress response related cis- acting elements, which may play a role in transcription and expression regulation of SoNIN1 gene. According to phylogenetic analysis and exonintron gene structure, the NIN proteins can be classified into 2 classes’type I and type II.

Key words: Sugarcane, Alkaline/neutral invertase, Exon Intron, Information analysis

Niu Junqi,Wu Chaoxing,Yang Litao,Li Yangrui. Analysis of Genomic Structure and Introns of SoNIN1 Gene from Sugarcane[J]. Biotechnology Bulletin, 2014, 0(12): 147-152.

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