Detection Methods of Genome-wide DNA Methylation Level Based on High-throughput Sequencing Technology

doi:10.13560/j.cnki.biotech.bull.1985.2015.07.007

Abstract

Abstract:

DNA methylation has been being the research focus of epigenetics study in the recent years. There are over 10 kinds of methods for detecting DNA methylation level, and detection of methylation status on genome-wide level has been achieved with the development of the NGS(Next-Generation Sequencing). At present the major detection methods of DNA methylation level on genome-wide scope based on NGS include BSP-seq(Bisulfite Sequencing PCR), MeDIP-seq(Methylated DNA Immunoprecipitation Sequencing)and MBD-seq(Methylated DNA Binding Domain Sequencing). In this paper, the research progress of principles, procedures, advantages and disadvantages, optimization of the usage and some bioinformatics resources on these 3 methods were reviewed, which aims to provide some beneficial ideas to researchers who are studying DNA methylation models while employing methods of high-throughput sequencing.

Key words: high-throughput sequencing technology, genome-wide, DNA methylation level, BSP-seq, MeDIP-seq, MBD-seq

Ma Langlang, Liang Zhenjuan, Xian Xin, Luo Shiwen, Li Qingchao, Liang Qianyun. Detection Methods of Genome-wide DNA Methylation Level Based on High-throughput Sequencing Technology[J]. Biotechnology Bulletin, 2015, 31(7): 45-50.

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