Detection of the RYR1 Gene in Guizhou Indigenous Pigs and Three-crossbred Pigs

doi:10.13560/j.cnki.biotech.bull.1985.2015.08.010

Biotechnology Bulletin ›› 2015, Vol. 31 ›› Issue (8): 66-70.doi: 10.13560/j.cnki.biotech.bull.1985.2015.08.010

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Detection of the RYR1 Gene in Guizhou Indigenous Pigs and Three-crossbred Pigs

Feng Wenwu¹, Long Weihai¹, Ding Mei³, Chen Xiang^{1, 2}, Chen Cunnian²

1. Key Laboratory of Animal Genetics,Breeding and Reproduction in the Plateau Mountainous Region,Ministry of Education,College of Animal Sciences,Guizhou University,Guiyang 550025; 2. Guizhou Engineering Technology Research Center for Healthy Pig Breeding,Guiyang 550025; 3. Guizhou Husbandry Animal Science Vocational School,Guiyang 550018

Received:2014-09-22 Online:2015-08-21 Published:2015-08-22

Abstract

Abstract: To provide the technical support for the cultivation of "strain of specialization with anti-stress and sensitive gene" of Guizhou local pig genes, the RYR1 gene in 151 samples from 5 local pig species of Baixi, Qianbei Black, Zongdi Hua, Nuogu, Congjiang Xiang and three-crossbred commercial pig were detected by PCR-RFLP technique. The pigs with HalNn of recessive anti-stress and sensitive genotype, HalNN of anti-stress and sensitive genotype and Halnn of stress and sensitive genotype were screened out. The results showed that all of 5 Guizhou indigenous pigs belonged to HalNN , there was no Halnn gene in the three-crossbred pigs, HalNn accounted for 52.63 % of the samples, and Haln allele frequency was 26.32%.

Key words: halothane gene, detection of RYR1 gene, fertility, PSE pork, meat quality

Feng Wenwu, Long Weihai, Ding Mei, Chen Xiang, Chen Cunnian. Detection of the RYR1 Gene in Guizhou Indigenous Pigs and Three-crossbred Pigs[J]. Biotechnology Bulletin, 2015, 31(8): 66-70.

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Detection of the RYR1 Gene in Guizhou Indigenous Pigs and Three-crossbred Pigs

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