Screening the Extracts of Marine Fungi for the Activity of Damaging the DNA of Escherichia coli and Researches on Active Strains

doi:10.13560/j.cnki.biotech.bull.1985.2015.08.020

Abstract

Abstract: Screening of antimicrobial and antitumor agents from marine microorganisms is one of the hot spots for the research of marine drugs, and screening models play an important role in the study. The authors set up an Escherichia coli differentiated DNA damage repair test model, which can be used for the screening of antimicrobial and potential antitumor substances. This model was further applied to screen the bioactivities of the extracts of marine symbiotic fungi. As the result, five marine fungal strains’ extracts showed strong selective inhibition to E. coli strains with DNA damage repairing genes-deficiency, i.e, the potential of damaging DNA. The five strains were identified by molecular biological methods. Furthermore, analyses by thin layer chromatography（TLC）and high performance liquid chromatography（HPLC）-bioactivity tracing revealed that these strains produced diverse bioactive substances.

Key words: marine microorganisms, Escherichia coli differentiated DNA damage repair test model, identification

Bao Hanyan, Gu Pengjuan, Zhang Yi, Mu Jun, Feng Yan, Zhao Chenyan. Screening the Extracts of Marine Fungi for the Activity of Damaging the DNA of Escherichia coli and Researches on Active Strains[J]. Biotechnology Bulletin, 2015, 31(8): 132-139.

References

[1] Buckingham J. Dictionary of Natural Products on DVD. Chapman and Hall/CRC, London, UK, 2011.
[2] Blunt JW, Copp BR, Keyzers RA, et al. Marine Natural Products [J]. Natural Product Report, 2014, 31：160-258.
[3] Blunt JW, Copp BR, Keyzers RA, et al. Marine Natural Products [J]. Natural Product Report, 2013, 30：237-323.
[4] 高传柱, 王天帅, 陈佳, 等. 铂类抗肿瘤药物作用机制研究进展[J]. 昆明理工大学学报, 2014, 39（4）：83-92.
[5] 李根, 林昱. DDRT法筛选抗肿瘤海洋微生物[J]. 中国抗生素杂志, 2004, 29：449-451.
[6] 许文思, 彭剑, 王吉成, 等. 一种新的快速简便的抗肿瘤药物筛选模型[J]. 中国抗生素志, 2001, 26（1）：15-18.
[7] Alam MZ, Ahmad S, Malik A. Genotoxic and mutagenic potential of agricultural soil irrigated with tannery effluents at Jajmau（Kanpur）, India[J]. Arch Environ Contam Toxicol, 2009, 57：463-476.
[8] ZhangY, Mu J, Feng Y, et al. Broad-spectrum antimicrobial epiphytic and endophytic fungi from marine organisms：isolation, bioassay and taxonomy[J]. Marine Drugs, 2009, 7（2）：97-112.
[9] 苏煦, 张翼, 穆军, 等. 海洋真菌抗病原菌生物活性物质的研究[J]. 天然产物与开发, 2011, 23：1025-1030.
[10] Harrison CJ, Langdale JA. A step by step guide to phylogeny reconstruction[J]. The Plant Journal, 2006, 45：561-572.
[11] Singh MP, Janso JE, Brady SF. Cytoskyrins and cytosporones produced by Cytospora sp. CR200：taxonomy, fermentation and biological activities[J]. Marine Drugs, 2007, 5：71-84.
[12] Zhang Y, Han JY, Mu J, et al. Bioactivity and constituents of several common seaweeds[J]. Chinese Science Bulletin, 2013, 58：2282-2289.
[13] 郭文, 蒋继志. 利用稻瘟霉模型初级筛选抗肿瘤物质研究进展[J]. 生物学通报, 2010, 45（4）：1-2.
[14] 李根, 陈瑞川, 林昱. 具抗肿瘤活性的海洋微生物菌株的初步筛选[J]. 台湾海峡, 2002, 21（1）：18-22.
[15] 邹国林, 周晖. 博莱霉素与DNA的作用机理及其类酶性研究[J]. 武汉大学学报, 1998, 44（6）：749-753.
[16] 贾金海, 李勇, 张晓琳, 等. 丝裂霉素对体外胃癌细胞DNA损伤和增殖的影响[J]. 肿瘤防治研究, 2012, 39（9）：1060-1063.
[17] 冯硕, 李正平, 张淑红, 等. 阿霉素和DNA相互作用的研究初探[J]. 河北职工医学院学报, 2004, 21（2）：43-44.
[18] 刘永辉, 李公春, 崔娇娇. 5-氟尿嘧啶类抗肿瘤药物的研究进展[J]. 河北化工, 2008, 31（9）：10-14.
[19] 王世卿. 紫杉醇类化合物抗肿瘤作用机制研究[J]. 淮海医药, 2008, 26（4）：375-376.
[20] Oshige M, Takenouchi M, Kato Y, et al. Taxol derivatives are sele- ctive inhibitors of DNA polymerasea α[J]. Bioorganic & Medici-nal Chemistry, 2004, 12（10）：2596-2601.
[21] 陈颖, 王磊, 王子健. 用彗星实验技术检测环境遗传毒性物质[J]. 土壤学报, 2006, 43（4）：673-678.