Selection and Identification of Oil-producing Energy Microalgae in Northeast Region

doi:10.13560/j.cnki.biotech.bull.1985.2015.08.035

Abstract

Abstract: In the 21st century, the ideal microalgae biodiesel fuel has been widespread concern, but how to filter high in fat content from a lot of microalgae species has become a problem to be addressed. In this study, 93 strains of microalgae were isolated and purified from the water samples of the Northeast region, and 30 strains were screened to obtain eight oil-producing algae by Nile red fluorescence. Oil production capacity of these eight microalgae were evaluated with bubble column photobioreactor. This experiment obtained a higher oil yield algal and its total oil yield reached 133. 9 mg /（L · d）. On this basis an oil-producing energy microalgae was identificated by 18S rRNA. This oil-producing energy microalgae was identificated as Chlorella sp.

Key words: oil-producing microalgae, nile red, separation and purification, screening, lipid productivity, molecular identification, 18S rRNA

Shi Wenjing, Liao Sha, Sun Qimei, Wang Pengxiang, Li Xiaoshu. Selection and Identification of Oil-producing Energy Microalgae in Northeast Region[J]. Biotechnology Bulletin, 2015, 31(8): 140-146.

References

[1] Breena L, Owende P. Biofuels from microalagae-A review of technologies for production, processing, and extrctions of biofuels and co-products[J]. Renew Sustain Energy Rev, 2010, 14：557-577.
[2] Hu Q, Sommerfeld M, Jarvis E, et al. Microalgal triacylglycerols as feedstorks for biofuel production：：perspectives and advances[J]. Plant J, 2008, 54：621-639.
[3] Ma F, Hanna MA. Biodiesel production：a review[J]. Bioresource Technol, 1999, 70：1-15.
[4] Turner JA. A realizable renewable energy future[J]. Science, 1999, 285（5427）：687-689.
[5] 郑洪立, 张齐, 马小琛, 等. 产生物柴油微藻培养研究进展[J]. 中国生物工程杂志, 2009, 29（3）：110-116.
[6] 梅洪, 张成武, 李夜光, 等. 利用微藻生产可再生能源研究概况[J]. 武汉植物学研究, 2008, 26（6）：650-660.
[7] Sheehan J, Dunahay T, Benemann J, et al. A look back at the US department of energy’s aquatic species program：biodiesel from algae[M]. US. ：National Renewable Energy Laboratory, 1998：1-64.
[8] 张齐, 郑洪立, 唐小红, 等. 基于模糊综合评价的产生物柴油微藻藻种筛选[J]. 中国生物工程杂志, 2010, 30（5）：69-75.
[9] Pedroni P, Davison J, Beckert H, et al. A proposal to establish an international network on biofixation of CO 2 and greenhouse gas abatement with microalgae[R]. Canada：IEA Greenhouse Gas R and D Program Executive Committee, 2002.
[10] Huang GH, Chen F, Wei D, et al. Biodiesel production by microal-gal biotechnology[J]. Applied Energy, 2010, 87：38-46.
[11] Rodolfi L, Chini Zittelli G, Bassi N, et al. Microalgae for oil：strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor[J]. Biotechnol Biotechnol Bioeng, 2009, 102（1）：100-112.
[12] Mandotra SK, Kumar P, Suseela MR, et al. Fresh water green microalga Scenedesmus abundans：A potential feedstock for high quality biodiesel production[J]. Bioresource Technology, 2014, 156：42-47.
[13] 蒋霞敏, 郑亦周. 14种微藻总脂含量和脂肪酸组成研究[J]. 水生生物学报, 2003, 27（3）;243-247.
[14] 王金娜, 严小军, 周成旭, 等. 产油微藻的筛选及中性脂动态积累过程的检测[J]. 生物物理学报, 2010, 26（6）：472-480.
[15] 王立柱, 温皓程, 等. 产油微藻的分离、筛选及自养培养氮源、碳源的优化[J]. 微生物学通报, 2010, 37（3）：336-341.
[16] 张秋会, 马莺. 工业化生产EPA和DHA藻株的选育[J]. 中国油脂, 2004, 29（6）：30-32.
[17] 张秋会, 马莺. 气相色谱法测定海洋微藻中二十碳五烯酸和二十二碳六烯酸[J]. 色谱, 2004, 22（6）：662.
[18] 易翠平, 杨明毅. 三种绿藻总脂肪和脂肪酸的测定[J]. 食品与机械, 2001（2）：36.
[19] 俞建江, 李荷芳, 周汉秋. 10种海洋微藻总脂、中性脂和极性脂的脂肪酸组成[J]. 水生生物学报, 1999, 23（5）：481-488.
[20] 卢丽娜, 孙利芹, 王长海, 等. 32株海洋微藻总脂含量及其脂肪酸组成的研究[J]. 中国油脂, 2009, 34（11）：68-73.
[21] 缪晓玲, 吴庆余. 藻类异养转化制备生物油燃料技术[J]. 可再生能源, 2004（4）：41-44.
[22] Miyamoto K. Renewable biological systems for alternative sustaina-ble energy production[R]. US：FAO Agricultural Services Bulletin, 1997：135.
[23] Rodolfi L, Zittelliv CG, et al. Microalgae for oil：strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor[J]. Biotechnol Bioeng, 2009, 102（1）：100-112.
[24] 李涛, 李爱芬, 桑敏, 等. 富油能源微藻的筛选及产油性能的评价[J]. 中国生物工程杂志, 2011, 31（4）：98-105.
[25] Richmond A. Handbook of microalgal culture：Biotechnology and applied phycology[M]. UK：Blackwell, 2004.
[26] 王海英, 符茹, 黄宝祥. 基于尼罗红荧光染色的小球藻脂质快速检测方法研究[J]. 中国油脂, 2012, 37（3）：78-81.
[27] 张桂艳, 温小斌, 梁芳, 等. 重要理化因子对小球藻（Chlorella）生长和油脂产量的影响[J]. 生态学报, 2011, 31（8）：2076-2085
[28] 黄建忠, 江贤章. DHA 高产菌Schizochytrium sp. FJU-512 的分离及其18S rRNA 基因序列比较分析[J]. 应用与环境生物学报, 2005, 11（2）：202-207.
[29] 王金娜, 严小军, 周成旭, 等. 产油微藻的筛选及中性脂动态积累过程的检测[J]. 生物物理学报, 2010, 26（6）：472-480.
[30] 万文文, 岳燕燕, 梁科鹏. 两株地下海水微藻的分子鉴定及其油脂分析[J]. 海洋环境科学, 2013, 32（3）：440-443.
[31] Alonzo F, Mayzaud P. Spectrofluorometric quantification of neutral and polar lipids in zooplankton using Nile red[J]. Marine Chemistry, 1999, 67（3-4）：289-301.
[32] 江丽丽, 温小斌, 耿亚红, 等. 一株产油微藻的筛选和分子鉴定[J]. 水生生物学报, 2013, 37（4）：606-612.
[33] 孙漫, 聂娟, 袁维道, 等. 产油脂海洋微藻的筛选、鉴定及Fe 3+ 对其生长和油脂积累的影响[J]. 微生物油脂, 2012, 37（12）：70-73.
[34] 周文俊, 郑立, 韩笑天, 等. 基于尼罗红染色分析金藻总脂动态积累[J]. 水生生物学报, 2014, 38（2）：312-319.