Isolation，Screening and Identification of Bacteria Antagonistic to Colletotrichum fructicola

doi:10.13560/j.cnki.biotech.bull.1985.2015.09.022

Abstract

Abstract: It is aimed to obtain a bacterial strain that has strongly antagonistic activity to Colletotrichum fructicola. Collecting rhizosphere soil from different superior individual of Sandalwood in different parts of Chengmai Forest Farm in Hainan Province and using dilution culture method, 71 strains of bacteria were isolated. Then using C. fructicola as the indicating bacterium, primary screening by flat confrontation and re-screening by fermentation liquid, one strain of bacterium with promising antagonistic activity was obtained, numbered as TXJ2-6. Antibacterial spectrum tests showed it had significantly antagonistic activities to 5 plant pathogens(Colletotrichum gloeosporioides, Agaricodochium camellia, Fusarium proliferatum, Pestalotiopsis microspora, and Colletotrichum gloeosporioides). Based on the morphological, physiological and biochemical characteristics and 16S rDNA phylogenetic analysis, strain TXJ2-6 was identified as Bacillus methylotrophicus.

Key words: Santalum album L., anthracnose, antagonistic bacteria, screening, identification

Tian Yuanyuan, Zhou Guoying, Zuo Jie, Liu Qianli, Yang Quan. Isolation，Screening and Identification of Bacteria Antagonistic to Colletotrichum fructicola[J]. Biotechnology Bulletin, 2015, 31(9): 158-162.

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