The Expression and Immune Response Characteristics of TLR2 at the Early Development Stage of Rana chensinensis

doi:10.13560/j.cnki.biotech.bull.1985.2015.10.001

Abstract

Abstract: It was to study the expression and immune response characteristics of TLR2 at the early development stage of Rana chensinensis. Immunohistochemistry and RT-PCR were used to locate and quantify the TLR2 mRNA from stage 11 to stage 42 of Rana chensinensis. Tadpoles at stage 29 were injected with antigen LTA, LPS, ZymoA and Poly I:C intraperitoneally. The expression changes of TLR2 mRNA were detected by RT-PCR within 24 h. TLR2 is widely distributed at the early development stage of Rana chensinensis. The relative expression of TLR2 mRNA increased form 0.187±0.021 to 0.373±0.031 at stage 25, and then returned to 0.170±0.020 progressively. The expression of TLR2 mRNA had no significant change in the LPS group and the Zymo A group（P>0.05）. TLR2 mRNA of the LTE group peaked twice at 4 h与12 h after antigen injection（P<0.01）; and the PolyI：C group peaked at 8 h（P<0.01）. The expression character of TLR2 matches with its living environment at the early development stage of Rana chensinensis.TLR2 did not responses to the challenge of LPS and ZymoA timely at the stage 29, on the other hand, TLR2 recognized and responsed to LTA and PolyI:C group effectively. TLR2 of Rana chensinensis possesses the recognition and response characteristics to PAMPs both of fish and mammals.

Key words: Rana chensinensis, TLR2, immune response, pathogen-associated molecular patterns

Wang Ze, He Tianyi, Lu Yuyan. The Expression and Immune Response Characteristics of TLR2 at the Early Development Stage of Rana chensinensis[J]. Biotechnology Bulletin, 2015, 31(10): 230-235.

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