Cloning and Expression of Outer Membrane Protein OprM from Aeromonas hydrophila and the Evaluation of Its Immunoprotective Effect

doi:10.13560/j.cnki.biotech.bull.1985.2019-0319

Abstract

Abstract: Aeromonas hydrophila is a common conditional pathogen. Controlling the damage of this bacterium in aquaculture industry is an urgent problem to be solved. Hereby the purified protein of A. hydrophila ATCC 7966 OprM was obtained by molecular biology methods such as cloning and expression. After intramuscular injection of the purified protein to zebrafish,the transcription level of immune-related genes was detected and the immune response was explored. The results showed that most of the immune-related genes were up-regulated,indicating that OprM protein evoked a considerable immune responses in zebrafish. Further velogenic strain A. hydrophila LP-2 was used to challenge the immunized zebrafish,and it was revealed that the relative immune protection rate after the injection of OprM protein reached 89.47%,the above results indicate outer membrane protein OprM is highly immunoprotective against zebrafish and is a potential vaccine candidate for A. hydrophila.

Key words: Aeromonas hydrophila, outer membrane protein OprM, immunogenicity, immune response

MAO Ran-ran, LI Xiao-yan, WU Yao, ZHANG Li-shan, LIN Zhen-ping, LIN Xiang-min. Cloning and Expression of Outer Membrane Protein OprM from Aeromonas hydrophila and the Evaluation of Its Immunoprotective Effect[J]. Biotechnology Bulletin, 2019, 35(9): 244-248.

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