Biotechnology Bulletin ›› 2015, Vol. 31 ›› Issue (10): 105-110.doi: 10.13560/j.cnki.biotech.bull.1985.2015.10.018

• Technique • Previous Articles     Next Articles

Virus-induced Gene Silencing and Its Application in Plant Research

Sun Wei, Xu Yi, Xu Guiying, Sun Peiguang, Song Shun, Chang Shenghe   

  1. Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Haikou 570102
  • Received:2014-11-20 Online:2015-10-28 Published:2015-10-28

Abstract: Virus-induced gene silencing(VIGS)is a useful tool for the identification of gene function in plants, therefore widely utilized in plants. Compared to traditional gene disruption approaches, VIGS is simple, rapid, short-cycle, transformation-free and capable of silencing multiple gene family members. Moreover, under the certain conditions, VIGS can even be transmitted to progeny plants. Here, the principle and operation of VIGS are introduced, and its recent applications and research advances are reviewed, which conducively promotes its further application and development in plants, especially for crop breeding.

Key words: virus-induced gene silencing, identification of gene function