Application of Mass Spectrometric Technology in DNA Methylation Study

doi:10.13560/j.cnki.biotech.bull.1985.2015.11.012

Abstract

Abstract: DNA methylation is one of most important epigenetic modifications, which mainly occurs in carbon-5 position of CpG nucleotide sequence in mammal genomes and relates to organism development, transposable silence, X chromosome inactivation and many diseases. The understanding for molecular mechanism of DNA methylation formation, maintenance and reconstruction remain limited. however, in order to find an optimal method for DNA methylation qualitative and quantitative analysis, mass spectrometric technology with high sensitivity, good performance, high precision, high resolution and good repeatability, has a great value in the study of DNA methylation. In the following, we will make a introduction of mass spectrometric technology application in whole genomic DNA methylation and promoter DNA methylation study.

Key words: whole-genomic DNA methylation, promoter methylation, mass spectrum, liquid chromatography

Huang Xinfeng, Ye Jinbo, Liu Jianjun. Application of Mass Spectrometric Technology in DNA Methylation Study[J]. Biotechnology Bulletin, 2015, 31(11): 112-120.

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