Expression Analysis of lc3b Gene in Gonadal Development of Triploid Female Rainbow Trout

doi:10.13560/j.cnki.biotech.bull.1985.2016-1166

Abstract

Abstract: This work aims to show a new light on the relationship between autophagy and gonadal regression of triploid female rainbow trout. Autophagy gene lc3b of rainbow trout was cloned by RT-PCR，and its expressions in gonads of triploid female rainbow trout were analyzed at different developmental period in mRNA and protein level. Gonadal ultrastructures were observed under transmission electron microscopy. Results showed clear evidence that lc3b gene was highly expressed in gonads of triploid female rainbow trout during the period of 200dpf to 270 dpf，in which autophagosome structures were identified. In this stage，the conversion of LC3B-I to LC3B-II was greater. These results demonstrated autophagy played pivotal roles in gonadal regression of triploid female rainbow trout.

Key words: triploid, rainbow trout, autophagy, LC3B, gonadal development

HUANG Tian-qing, WANG Xing-ran ,WANG Yan-na, SUN Hui-zhi, HAN Ying. Expression Analysis of lc3b Gene in Gonadal Development of Triploid Female Rainbow Trout[J]. Biotechnology Bulletin, 2017, 33(7): 179-184.

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