Abstract: The objective of this work is to investigate the effect of collagen scaffolds combined with salidroside microspheres on rat bone mesenchymal stem cells(rBMSCs). The salidroside was made into microspheres that combined into the collagen. The surface character of material was observed by scanning electron microscopy(SEM). The material was implanted with rBMSCs and observed for the cell adhesion on the material by SEM. The cell proliferation on the material was measured by CCK-8, and the cell proliferation and morphology on the material were detected by HE staining. S100 immunofluorescence was used to detect the differentiation of stem cells into nerve cells. Cells were seeded onto the scaffold, adhered and grew well on materials. While the time increased, the cells on the same material were significant proliferation(P < 0.05). The expression of nerve cell marker protein S100 was positive. Conclusively, collagen combined with salidroside microspheres had good biocompatibility, salidroside microspheres did not affect cell proliferation on the material, and it could induce effectively rBMSCs into nerve cells.

Key words: salidroside, collagen, scaffold, bone marrow mesenchymal stem cells