The Identification and Analysis of Sulfate-assimilation Related Genes in  Acidithiobacillus ferrooxidans

doi:10.13560/j.cnki.biotech.bull.1985.2016.02.017

Abstract

Abstract: This work aims to identify and analyze the related genes of sulfate-assimilation in Acidithiobacillusferrooxidans ATCC 23270. Firstly, the genes involved in sulfate-assimilation in the genome of A.ferrooxidans ATCC 23270 were analyzed using bioinformatics.Then we co-transcripted and identified the candidate genes cys JI, cys H, cys D-2, cys N, cys D-1, and cys NC probably involving in encoding cysteine(Cys)operon by reverse transcription and gel electrophoresis.Further we cloned in vitro, expressed, purified and recombined the key genes, and confirmed their functions and measured their enzyme activities.Results showed that among these candidate genes of Cys operon, cys D-1 and cys NC were co-transcripted, and Cys JI, cys H, cys D-2 and cys N were co-transcripted.The possible basic metabolic pathway of sulfate-assimilation in A.ferrooxidans ATCC 23270 was determined preliminarily, and the regulatory mechanism of Cys operon was explored.

Key words: Acidithiobacillus ferrooxidans, sulfate-assimilation, co-transcription

ZHENG Chun-li, WANG Dan, ZHANG Li, CHEN Min-jie, MA Hong-wei, JIANG Zhi-yan. The Identification and Analysis of Sulfate-assimilation Related Genes in Acidithiobacillus ferrooxidans[J]. Biotechnology Bulletin, 2016, 32(2): 131-139.

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The Identification and Analysis of Sulfate-assimilation Related Genes in Acidithiobacillus ferrooxidans

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