The Relationship Between Characteristics of Yielding Carotenoid and Expression of Carotenogenic Genes in Phaffia rhodozyma

doi:10.13560/j.cnki.biotech.bull.1985.2016.02.016

Abstract

Abstract: In order to study the metabolic regulation mechanism of carotenoid synthesis in Phaffia rhodozyma, the carotenoid biosynthesis characteristics and the expression differences of carotenogenic genes in different strains of P.rhodozyma, JMU-VDL668 and JMU-N3 were analyzed by HPLC and real-time qPCR, respectively.The effects of adding β-carotene during the culture of strain JMU-VDL668 to the yield of astaxanthin were also analyzed.The results showed that, the expression levels of the carotenogenic genes crtE, crtI, crtYB and crtS of key enzymes in the metabolic pathway of carotenoid in P.rhodozyma were lower in JMU-VDL668 than JMU-N3, even the expression of crtR was not detected in JMU-N3.The synthesis of astaxanthin increased when β-carotene was added at 72 h and 96 h of culturing JMU-VDL668, and the astaxanthin increased 83% while adding β-carotene at 96 h compared to the control group.The results indicated that the characteristics of yielding carotenoid was positively correlated to the expression level of carotenogenic genes.Moreover, the productivity of cells for total carotenoid in JMU-VDL668 was in a certain range, suggesting that there was a feedback regulation.

Key words: Phaffia rhodozyma, astaxanthin, real-time qPCR, metabolic regulation

ZHENG Chen-hua, DU Xi-ping, LI Li-jun, LI Tian-li, CAO Ying, NI Hui. The Relationship Between Characteristics of Yielding Carotenoid and Expression of Carotenogenic Genes in Phaffia rhodozyma[J]. Biotechnology Bulletin, 2016, 32(2): 123-130.

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