Isolation and Identification of a High-temperature Resistant Bacterium CICC 10853

doi:10.13560/j.cnki.biotech.bull.1985.2016.02.018

Abstract

Abstract: In this study, we identified the high-temperature resistant bacterium CICC 10853 isolated from slant culture.The strain was identified and characterized via morphological features, physiological and biochemical characteristics, 16S rRNA and recN gene sequence analysis.According to the results, strain CICC 10853 was identified as Geobacillus thermoleovorans, which lays the foundation for the further study of biological function of the bacterium.

Key words: thermophilic bacterium, CICC 10853, identification

DU Hai-bo, ZHANG Xin, LI Zan, LIU Yi, LIU Bo, LI Jin-xia, YAO Su, CHENG Chi. Isolation and Identification of a High-temperature Resistant Bacterium CICC 10853[J]. Biotechnology Bulletin, 2016, 32(2): 140-145.

References

[1] 崔宗均, 路鹏, 于会泳, 张晋丰.高温菌生物学特性的应用[J].微生物学杂志, 2003(4)：28-31.
[2] 曹军卫, 沈萍, 李朝阳.嗜极微生物[M].武汉：武汉大学出版社, 2004.
[3] 潘丽贞, 陆利霞, 熊晓辉.嗜热菌产酶现状的研究[J].食品工业科技, 2012(3)：422-424.
[4] 和致中, 彭谦, 陈俊英.高温菌生物学[M].北京：科学出版社, 2001.
[5]东秀珠, 蔡妙英.常见细菌系统鉴定手册[M].北京：科学出版社, 2001.
[6]张欣, 刘洋, 姚粟, 等.1株分离自西沙诺尼果原浆细菌菌株CICC10881的鉴定及生物学特性研究[J].食品与发酵工业, 2014, 40(11)：27-32.
[7] Kim OS, Cho YJ, Lee K, et al.Introducing EzTaxon：a prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species[J].Int J Syst Evol Microbiol, 2012, 62：716-721.
[8]Thompson JD, Higgins DG, GibsonTJ.CLUSTAL W：improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice[J].Nucleic Acids Res, 1994, 22：4673-4680.
[9]Tamura K, Dudley J, Nei M, et al.MEGA4：Molecular Evolutionary Genetics Analysis(MEGA)software version 4.0[J].Molecular Biology and Evolution, 2007, 24：1596-1599.
[10] Zeigler DR.Application of a recN sequence similarity analysis to the identification of species within the bacterial genus Geobacillus[J].Int J Syst Evol Microbiol, 2005, 55(3)：1171-1179.
[11] De Vos P, Garrity GM, Jones D, et al.Bergey’s manual of systematic bacteriology, second edition, volume three[M].East Lansing：Michigan State University, 2009.
[12]Kim M, Oh HS, Park SG, Chun J.Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes[J].Int J Syst Evol Microbiol, 2014, 64：346-351
[13] Dinsdale AE, Halket G, Coorevits A, et al.Emended descriptions of Geobacillus thermoleovorans and Geobacillus thermocatenulatus[J].Int J Syst Evol Microbiol, 2011, 61：1802-1810.
[14] Nazina TN, Tourova TP, Poltaraus AB, et al.Taxonomic study of aerobic thermophilic bacilli：descriptions of Geobacillus subterra-neus gen.nov., sp.nov.and Geobacillus uzenensis sp.nov.from pet-roleum reservoirs and transfer of Bacillus stearothermophilus, Baci-llus thermocatenulatus, Bacillus thermoleovorans, Bacillus kausto-philus, Bacillus thermoglucosidasius and Bacillus thermodenitrifi-cans to Geobacillus as the new combinations G.stearothermophilus, G.[J].International Journal of Systematic and Evolutionary Microbiology 2001, 51：433-446.
[15] Marchant R, Banat IM.TheGenus Geobacillus and Hydrocarbon Utilization//Handbook ofHydrocarbonand Lipid Microbiology[M].Springer Berlain Heidelberg, 2010：1887-1896.
[16]http://www.straininfo.net/taxa/3481/browser/euzeby