The Establishment of HepG2 Cell Line with TALEN-mediated Knockout of CXCR4

doi:10.13560/j.cnki.biotech.bull.1985.2016.02.031

Abstract

Abstract: It was to research on the effect of CXCR4 on HepG2 cells, we established one cell line which could down-regulate CXCR4 expression stably by transcription activaor-like effector nuclease (TALEN) gene targeting.The hepatocellular carcinoma cell line HepG2 was chosen for this study, and the expression of CXCR4 was interfered by transcription activator-like effector nuclease (TALEN).The constructed CXCR4 TALEN plasmids were transfected into HepG2 cells, and the targeting efficiency was determined as 40% by enzyme digestion of T7E1.The single clone cell of CXCR4 knockout was screened by gene sequencing, and the significant decrease of gene CXCR4 expression was confirmed by immunofluorescence and Western blot.The hepatocellular carcinoma cell line with stably down-regulating CXCR4 expression was established successfully by TALEN gene targeting.

Key words: TALEN, CXCR4, hepatocellular carcinoma cell, gene target

ZHANG Wen-mei, DING Yan, GUO Xing-rong, LI Dong-sheng, ZHAO Wan-hong, WANG Xiao-li. The Establishment of HepG2 Cell Line with TALEN-mediated Knockout of CXCR4[J]. Biotechnology Bulletin, 2016, 32(2): 225-228.

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