Research Progress in Genome Editing of Lepidoptera Insects

doi:10.13560/j.cnki.biotech.bull.1985.2019-0875

Abstract

Abstract: Insects of the order Lepidoptera are among the most diverse and species-rich groups of organisms. Gene function research through genome editing is an efficient and practical way to study the genetic,physiological and biochemical regulation mechanism. The technologies of zinc finger nucleases（ZFNs）,transcription activator-like effector nucleases（TALENs）and CRISPR/Cas（clustered regularly interspaced short palindromic repeats,CRISPR;CRISPR associated protein,Cas）are 3 major genome editing techniques applied in Lepidoptera insects genome editing. Their basic principle is as such：triggering DNA damage repair mechanism by making DNA double-strand breaks（DSBs）damages in the genome,and subsequently resulting in the mutations of inserting and deleting. In this paper,the basic principles of the ZFNs,TALENs and CRISPR/Cas technologies and research progresses on their applications in order Lepidoptera insects such as Bombyx mori,Spodoptera litura,Helicoverpa armigera are briefly overviewed. This provides references for functional genomics study,insect physiology and biochemistry,insect ethology and the establishment of green pest control system. Finally,the application status of genome editing technology in Lepidoptera insects is briefly summarized,and the establishment and application of Lepidoptera-specific gene editing technology are prospected.

Key words: ZFNs, TALENs, CRISPR/Cas, Lepidoptera, gene edit

CHENG Ying, JIN Ming-hui, XIAO Yu-tao. Research Progress in Genome Editing of Lepidoptera Insects[J]. Biotechnology Bulletin, 2020, 36(3): 18-28.

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