Biotechnology Bulletin ›› 2016, Vol. 32 ›› Issue (5): 172-179.doi: 10.13560/j.cnki.biotech.bull.1985.2016.05.023

Previous Articles     Next Articles

Isolation and Utilization of a Thifensulfuron-methyl-degrading Bacterium,Bacillus subtilis LXL-7

LI Xiao-lou   

  1. Department of Architecture and Environmental Engineering Department,Sichuan Vocational and Technological College,Suining 629000
  • Received:2015-09-14 Online:2016-05-25 Published:2016-05-27

Abstract: A strain LXL-7 isolated from soil highly degrades thifensulfuron-methyl,and was identified as Bacillus subtilis. The degradation rate by LXL-7 reached 99.6% in mineral salt medium of thifensulfuron-methyl(100 mg/L)after 72 h. The optimal pH value and temperature were 7.5 and 30-35℃. The strain tolerated well to thifensulfuron-methyl and salt,at least to 400 mg/L of thifensulfuron-methyl and 4.0% NaCl. In addition,adding strain LXL-7 in the commercial preparation for soil amendment,a new multiple-function microbial preparation was developed,and it had an additional new function of degrading thifensulfuron-methyl residue in soil except original functions. Therefore,the strain LXL-7 could be considered for bioremediation of thifensulfuron-methyl pollution and development of microbial preparation.

Key words: thifensulfuron-methyl, Bacillus subtilis, biodegradation, microbial preparation