Screening and Identification of Biocontrol Bacillus sp. Against Astragalus Root Rot and Its Pot Experiment

doi:10.13560/j.cnki.biotech.bull.1985.2019-0329

Abstract

Abstract: Astragalus root rot is one of the diseases that seriously affect the yield of astragalus. In order to obtain one bacillus strain with strong antagonistic activity against the pathogen of astragalus root rot,the pathogenic bacterium Fusarium solaniun KR997532 was used as the target strain,the antagonistic bacillus strains of the plant pathogens preserved in our laboratory were screened and rescreened,one bacillus strain 7-2-1-6 with strong antagonistic biological activity was obtained. Strain 7-2-1-6 was identified as Bacillus subtilis subsp. Spizizenii based on the 16S rDNA sequence analysis and the morphological characteristics and physiological and biochemical characteristics of strain 7-2-1-6. The bio-control effect of strain 7-2-1-6 achieved about 68.80% to astragalus root rot in pot experiment.

Key words: Astragalus membranaceus root rot, biological control, Bacillus subtilis

ZHAO Xiao-xia, NIU Shi-quan, WEN Na, SU Feng-feng. Screening and Identification of Biocontrol Bacillus sp. Against Astragalus Root Rot and Its Pot Experiment[J]. Biotechnology Bulletin, 2019, 35(9): 107-111.

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