Isolation and Identification of an Antagonistic Bacterial Strain Against Rice Blast Fungus

doi:10.13560/j.cnki.biotech.bull.1985.2016.06.024

Abstract

Abstract: It is quite important to develop novel and environment-safe strategies to control rice blast caused by Magnaporthe oryzae，and screening effective microorganism resources is primary basis for the bio-control of blast. Total 817 strains were isolated from healthy ones among diseased rice plants of the susceptible cultivar Xiangzaoxian 24，a single-season cropping rice，in the mountainous area of Taojiang，Hunan，in 2014. One strain JN005 with efficient antagonism to M. oryzae among 817 strains was screened by the plate confrontation method. The inhibition rate of metabolite from active bacterium to the rice blast reached 84% by measuring the growth rate of mycelium of the blast fungus. Further，antimicrobial spectrum of JN005 against 5 plant pathogenic fungi，4 plant pathogenic bacteria，and also Escherichia coli and Staphylococcus aureus sub. sp. aureus，was tested. The result revealed that JN005 also presented high inhibitory effect to Fusarium graminearum and Ustilaginoidea virens，and the inhibition rates to two fungi were 70.1% and 73.6%，respectively. Based on analysis of the morphological，physiological，and biochemical characteristics and the 16S rDNA phylogeny，strain JN005 was identified as Bacillus subtilis.

Key words: rice, Magnaporthe oryzae, antagonistic bacteria, isolation, identification

GUAN Ling-li, REN Zuo-hua, LI Jun-jun, LIU Er-ming. Isolation and Identification of an Antagonistic Bacterial Strain Against Rice Blast Fungus[J]. Biotechnology Bulletin, 2016, 32(6): 168-173.

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