Identification of Two Novel Phosphorylated Sites of Homeoprotein Msx1

doi:10.13560/j.cnki.biotech.bull.1985.2016.06.031

Abstract

Abstract: Some phosphorylated sites in the homolog Msx2 of the homeoprotein Msx1 has been identified，which plays a crucial biological role. However，the phosphorylated sites in Msx1 are not known yet，thus in order to examine the Msx1 phosphorylated status，we applied bioinformatics analysis to predict phosphorylation sites in Msx1，further used immunoprecipitation and mass spectrometry to experimentally verify the phosphorylation sites in Msx1. Tryptic digests of Msx1 protein complexes purified from C2C12 cells were separated and analyzed by nLC-MS-MS. Two novel phosphorylation sites(Ser152 and Ser160)were identified，moreover，these two sites were highly conserved in mouse and human. Furthermore，the specifically-phosphorylated antibody was prepared targeting these two phosphorylation sites.

Key words: homeoprotein, Msx1, phosphorylation, mass spectrometric identification

CHENG Qing-ling, WANG Jing-qiang. Identification of Two Novel Phosphorylated Sites of Homeoprotein Msx1[J]. Biotechnology Bulletin, 2016, 32(6): 211-218.

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