Clone and Expression of WalR in Streptococcus pneumonia TCSs and Analyzation of Conservation and Antigenic Epitope

doi:10.13560/j.cnki.biotech.bull.1985.2016.07.028

Abstract

Abstract: This work aims to express WalR protein in Escherichia coli BL21 through constructing recombinant expression vector PET28a-WalR and to analyze the immunogenicity in the experimental animal C57 mice,as well as to test the conservation in differentStreptococcus pneumonia serotypes and B cell antigenic epitopes. Using the WalR gene of S. pneumonia D39 as the template,the recombinant expression vector PET28a-WalR was constructed and then transferred into Escherichia coli BL21 for induced expressing target protein. The conservation of WalR protein in varied S. pneumonia serotypes was analyzed by ClustalX 2.1 software,and the B cell antigenic epitopes was analyzed by DNASTAR Lasergene v7.1. As result,recombinant expression vector PET28a-WalR was constructed successfully and much target protein was obtained by inducement of IPTG,however mainly in inclusion body. The conservation of WalR protein was up to 99.4% among different S. pneumonia serotypes,and the antigenic epitopes of WalR protein were likely located in 9-16,35-42,95-111,113-135,150-161,and 200-218 of amino acid sequence. In conclusion,recombinant S. pneumonia WalR protein in the form of inclusion body was successfully acquired,and the conservation and antigenic epitope were analyzed.

Key words: Streptococcus pneumonia, WalR, recombinant protein, conservation, antigenicity

HAN Dao-bin, LUO Shi-lu, HUANG Jian, ZHU Jie-hua, CHEN Ze-hui, MIN Xun. Clone and Expression of WalR in Streptococcus pneumonia TCSs and Analyzation of Conservation and Antigenic Epitope[J]. Biotechnology Bulletin, 2016, 32(7): 194-199.

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