Expression, Purification, Crystallization of SPD1587 Protein from Streptococcus pneumoniae

doi:10.13560/j.cnki.biotech.bull.1985.2015.07.035

Abstract

Abstract: SPD1587 protein is a transcription factor of streptococcus pneumoniae D39 strains, which plays an important role in nasopharyngeal colonization and lung infection. It has a conserved domain that was similar to transcription factors Mga of group A streptococcus, but the protein three-dimensional structure is unclear. In this study, the protein expression vector of PET28a-spd1587 was successfully constructed and a soluble form of SPD1587 protein was acquired in Escherichia coli BL21(DE3)strain. High purity SPD1587 protein was obtained by Ni-NTA affinity chromatography and DEAE negative ion exchange chromatography. The crystal of SPD1587 protein was obtained by the vapor diffusion method.

Key words: Streptococcus pneumoniae, SPD1587 protein, prokaryotic expression, crystal grow

Huang Jian, Huang Meirong, Luo Shilu, Zhu Jiehua, Min Xun. Expression, Purification, Crystallization of SPD1587 Protein from Streptococcus pneumoniae[J]. Biotechnology Bulletin, 2015, 31(7): 226-230.

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