Cloning of Small Heat-shock Protein（HSP）Gene from Sugarcane and Analysis of Its Expression Under Drought Stress

doi:10.13560/j.cnki.biotech.bull.1985.2016.10.020

Abstract

Abstract: The present experiment was conducted to clone Small Heat-shock Protein（sHSP）in sugarcane to explore mechanism of stress resistance in sugarcane. sHSP was amplified using RT-PCR from sugarcane leaves,the characteristics of the deduced protein were analyzed using bioinformatics software and its expression was analyzed using quantitative real-time PCR. The results showed that the cDNA of HSP gene was 659 bp in full length with a 459 bp open reading frame,and encodes a putative sHSP protein with 152 amino acids. Comparison of the amino acids sequences homology in sHSP protein from 14 different species indicated that,the sHSP protein had 69% to 96% identity in amino acids sequence with other plants. The deduced amino acids sequence not only contained a typical sHSP domain,but also was conservative. The results of quantitative real-time PCR analysis showed that the mRNA of sHSP was decreased initially and then increased with time under drought stress. These results suggested that sHSP might be involved in function of water stress and drought resistance in sugarcane enhanced by Si application.

Key words: sugarcane, drought, silicon, HSP

LIANG Pan-xia, HUANG Xing, LI Yang-rui. Cloning of Small Heat-shock Protein（HSP）Gene from Sugarcane and Analysis of Its Expression Under Drought Stress[J]. Biotechnology Bulletin, 2016, 32(10): 163-169.

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