Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (11): 238-251.doi: 10.13560/j.cnki.biotech.bull.1985.2023-0588

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Identification of AP Gene Family and Its Response Analysis to Abiotic Stress in Setaria italica

XING Yuan1,2(), SONG Jian3, LI Jun-yi1, ZHENG Ting-ting1,2, LIU Si-chen1,2(), QIAO Zhi-jun1,2()   

  1. 1. Center for Agricultural Genetic Resources Research, College of Agronomy, Shanxi Agricultural University, Taigu 030801
    2. Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau of the Ministry of Agriculture and Rural Affairs, Taiyuan 030031
    3. Agricultural Cash Crop Research Institute of Shanxi Agricultural University, Lvliang 032200
  • Received:2023-06-20 Online:2023-11-26 Published:2023-12-20
  • Contact: LIU Si-chen, QIAO Zhi-jun E-mail:xy15315514037@163.com;lsch@163.com;nkypzs@126.com

Abstract:

Aspartic protease(AP)is a pivotal hydrolase enzyme that assumes a critical role in plant growth, development, resilience against both biological and abiotic stressors. Foxtail millet(Setaria itlica)is the model crop for stress resistance study of C4 gramineous plants, but there are few studies on the gene function of AP(SiAPs)in foxtail millet. In order to further explore the functional role of the APs in foxtail millet, the members of the SiAPs were indentified based on whole-genome screening of SiAPs conserved Pfam sequences, and bioinformatics method was used to analyze their physical and chemical properties, subcellular localization, gene structure, conserved domain, phylogenetic development, promoter cis-acting elements and collinearity by bs. Meanwhile, fluorescence quantitative PCR technique was used to study its expression mode under abiotic stress. The results showed that there were 58 AP gene family members in foxtail millet genome. Phylogenetic tree analysis demonstrated that the gene family could be divided into 5 subfamilies, among which Group B encoded atypical AP, while other subfamilies encoded nucellin-like AP. The analysis of gene structure and conserved motifs revealed that members of the same subfamily of APs in foxtail millet were highly conserved. Collinearity analysis showed that there were a large number of homologous gene pairs between SiAPs and rice(Oryza sativa)and maize(Zea mays)AP genes. The study of promoter cis-acting elements indicated that most members of SiAPs contained cis-acting elements related to abiotic stress and biohormone response, such as cis-acting elements in response to drought and low-temperature stress, and salicylic acid response elements. Further quantitative PCR results uncovered that SiAPs were differentially expressed in the root, stem, leaf and panicle of foxtail millet. The expressions of SiAP3, SiAP9 and SiAP48 genes significantly increased under low temperature stress. Under drought stress and SA treatments, the change trend of some gene expression was basically the same. SiAPs play an important role in regulating foxtail millet response to abiotic stress. The results of this study may provide reference for stress resistance analysis of SiAPs.

Key words: foxtail millet(Setaria itlica), aspartate protease, salicylic acid response, drought response, low-temperature stress, bioinformatics