Biotechnology Bulletin ›› 2016, Vol. 32 ›› Issue (10): 199-204.doi: 10.13560/j.cnki.biotech.bull.1985.2016.10.024

• Orginal Article • Previous Articles     Next Articles

The Expression Differences Between Bacterial Blight Resistance Genes and Signal Materials in Embryonic Cell and Leaf of O.meyeriana Bail

LIU Lin1, PENG Bo2, JIANG Chun-miao3, 4, CHENG Zai-quan4   

  1. 1. Xinyang University,Xinyang 464000;
    2. Xinyang Normal University,Xinyang 464000;
    3. Yunnan University,Kunming 650091;
    4. Biotechnology﹠Genetic Resources Institute,Yunan Academy of Agriculture Sciences,Kunming 650223
  • Received:2016-03-30 Online:2016-10-25 Published:2016-10-12

Abstract: In order to research the effect of the Oryzae on the resistance signal substance and resistant bacterial blight gene of O.meyeriana Bai,the O.meyeriana embryonic cells(callus,specialized cells)and leaf cells(no-specialized cells)were treated with the Oryzae. Then the content change of resistance signal substance salicylic acid(SA)and jasmonic acid(JA),and the expression of genes related to bacterial blight resistance were detected. The research has shown that the content of SA and JA in callus before and after treatment was higher than that in leaf cells. This may be related to the cell wall of no-specialized callus which are thinner in order to prevent the invasion of pathogenic bacteria.In order to prevent the invasion of pathogenic bacteria,the expression was increased. At the same time,it shows that the ability to resist the Oryzae of the O.meyeriana is in the primary germ cell. The ME207 gene was expressed before and after the specialized differentiation of the O.meyeriana cells. But the ME281 gene is an inducible expression gene before the specialized differentiation of the O.meyeriana cells,which is a constitutive expression gene after the specialized differentiation of the O.meyeriana cells.

Key words: O. meyeriana, resistance signal substance, salicylic acid(SA), jasmonic acid(JA), resistant bacterial blight gene